Chinese herbal medicine germicide
A technology of Chinese herbal medicine fungicides and fungicides, applied in the direction of fungicides, biocides, biocides, etc., to achieve the effects of good disease prevention and treatment, stable drug efficacy, and convenient production, storage and transportation
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Embodiment 1
[0033] Example 1. Preparation of bactericide
[0034] Take 60 grams of Xanthium, 30 grams of Cork, 30 grams of Cnidium, 30 grams of Sophora flavescens, 60 grams of Alum, 15 grams of Honeysuckle, 15 grams of Honeysuckle, 15 grams of Ginkgo Leaf, 20 grams of Pulsatilla and 15 grams of Asarum, according to tradition Traditional Chinese medicine boiling method: After mixing, add 2000ml, 1000ml and 1000ml of water in sequence, and boil 3 times, each time to 200~300ml, the filtrate of 3 times is combined and heated and concentrated to 500ml to make a liquid, which is filtered by multiple layers of gauze. After the bacteria filter is filtered and sterilized, the bactericide LS-1 is obtained.
Embodiment 2
[0035] Example 2. Toxicity determination of fungicides
[0036] 1. Material
[0037] 1.1, test materials
[0038] Test strains Phytophthora sojae, Phytophthora capsici, Phytophthora capsici, Alternaria solani, Fusarium oxysporum Schlecht., Alternaria Alternaria alternata, Exserohilum turcicum, Botrytis cinerea, Fusarium graminicola and Pyricularia oryzae are all common commercial strains.
[0039] 1.2. Test medicine
[0040] 30% Fuweiling WP Shenzhen Rui Defeng Pesticide Co., Ltd.
[0041] 50% carbendazim WP Henan Mengzhou Pingyuan Pesticide Co., Ltd.
[0042] 58% Ruidu mold wettable powder, UK Noppe Group, Shenzhen Noppe Agrochemical Co., Ltd.
[0043] Thiophanate methyl WP produced by Haili Guixi Chemical Pesticide Co., Ltd.
[0044]Kelu WP E.1.DUPONT DE NEMOURS AND COMPANY
[0045] Allicin EC Shandong Jinan Chemical Plant
[0046] Fungicide LS-1
[0047] 2. Test method
[0048] Bacteriostatic determination-the growth rate method uses the bactericide LS-1 as the mother liquor, a...
Embodiment 3
[0053] Example 3 The effect of fungicides on the formation and germination of spores (cysts) of plant pathogens
[0054] 1. Test materials
[0055] Pseudoperonospora cubensis, Phytophthoracapsici, Fusarium oxysporum, Alternaria alternata, and Pyricularia oryzae are all commercial strains.
[0056] 2. Test method
[0057] The effect of the test agent on the germination of spores (capsules): mix the spores or sporangia of the tested plant pathogenic bacteria into 50-60 spores per 10 times the field of view, and then use the spore suspension to dilute the chemical agent, and test before dilution The chemical agent is prepared into a mother liquor of 150, and then the calculated amount of the medicine to be added in 2ml is diluted with the spore suspension to 2ml and added to the wells of the 24-well cell culture plate. The bacterial suspension without the agent is used as the control. Cover the lid and place it in a constant temperature incubator for 24h or 48h, investigate and calcu...
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