Chinese herbal medicine germicide
A technology of Chinese herbal medicine fungicides and fungicides, applied in the directions of fungicides, biocides, biocides, etc., to achieve the effects of stable drug efficacy, convenient production, storage and transportation, and good disease prevention and treatment effects.
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Embodiment 1
[0033] Embodiment 1, the preparation of fungicide
[0034] Take 60 grams of cocklebur, 30 grams of cortex, 30 grams of cnidium, 30 grams of flavescens, 60 grams of alum, 15 grams of honeysuckle, 15 grams of honeysuckle, 15 grams of ginkgo leaves, 20 grams of Pulsatilla chinensis and 15 grams of asarum. Boiling method of traditional Chinese medicine: After mixing, add 2000ml, 1000ml and 1000ml of water in turn, boil 3 times, boil each time to 200-300ml, combine the filtrates for 3 times, heat and concentrate to 500ml to make water, filter through multi-layer gauze Filter and sterilize with a bacterial filter to obtain the fungicide LS-1.
Embodiment 2
[0035] Embodiment 2, the toxicity determination of fungicide
[0036] 1. Materials
[0037] 1.1. Test materials
[0038] The tested strains Phytophthora sojae, Phytophthora capsici, Phytophthora capsici, Alternaria solani, Fusarium oxysporum Schlecht. Alternaria alternata), Exserohilum turcicum, Botrytis cinerea, Fusarium graminicola, and Pyricularia oryzae are common commercial strains.
[0039] 1.2. Drugs to be tested
[0040] 30% futhering spirit wettable powder Shenzhen Rui Defeng Pesticide Co., Ltd.
[0041] 50% carbendazim wettable powder Henan Mengzhou Pingyuan Pesticide Co., Ltd.
[0042] 58% Ruifu mold wettable powder United Kingdom Nuopai Group Shenzhen Nuopai Agrochemical Co., Ltd.
[0043] Thioprazine-methyl wettable powder produced by Haili Guixi Chemical Pesticide Co., Ltd.
[0044] Kelu wettable powder E.1.DUPONT DE NEMOURS AND COMPANY
[0045] Allicin EC Shandong Jinan Chemical Factory
[0046] Fungicide LS-1
[0047] 2. Test method
[0048] Determinati...
Embodiment 3
[0053] Embodiment 3, bactericide is to the influence of phytopathogenic bacteria spore (capsule) formation and germination
[0054] 1. Test materials
[0055] Pseudoperonospora cubensis, Phytophthora capsici, Fusarium oxysporum, Alternaria alternata, Pyricularia oryzae are all commercial strains.
[0056] 2. Test method
[0057] The effect of the test agent on the germination of spores (sacs): make the spores or sporangia of the plant pathogenic bacteria to be tested into 50-60 spores per 10 times field of view, and then dilute the chemical agent with the spore suspension. The chemical agent is made into 150 mother liquor, and then according to the calculated amount of drug to be added in 2ml, it is diluted to 2ml with spore suspension and added to the well of a 24-well cell culture plate. Cover and place in a constant temperature incubator for 24h or 48h and then investigate and calculate the spore germination rate.
[0058] The effect of the test agent on the formation of...
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