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Culture solution and preservation solution for coccidian oocyst

A technology of culture fluid and preservation fluid, which is applied in the field of culture fluid and preservation fluid of coccidiosis oocysts of chicken coccidiosis vaccine, and can solve problems affecting human and animal health, residues, etc.

Active Publication Date: 2006-09-27
FOSHAN STANDARD BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, potassium dichromate is a heavy metal compound, which will remain in animal bodies and the environment after long-term use, affecting human and animal health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Example 1 The effect of chloramine T on the development of coccidia oocyst spores

[0012] 1 Materials and methods

[0013] 1.1 Culture medium

[0014] Negative control (purified water), positive control (2.5% potassium dichromate solution), 0.5%, 1%, 2%, 4%, 8% chloramine T solution.

[0015] 1.2 Preparation of fresh oocysts

[0016] Infect 40 14-day-old healthy chicks with coccidia mixed oocysts at a dose of 10,000 oocysts / chicken, collect feces discharged within 6 hours during the peak period of oocyst ovulation on the 7th day after infection, and prepare the production process according to the coccidia vaccine Isolate and purify fresh coccidia oocysts.

[0017] 1.3 Test grouping and treatment

[0018] Divide the clean coccidia oocysts into 7 parts, suspend them in the 7 kinds of culture solutions designed above, make the culture density 80,000 oocysts / ml, and culture them in a 29°C incubator, every few hours Stir gently once. At 14h, 21h, 28h and ...

example 2

[0025] Example 2 Determination of Vitality of Coccidia Oocysts Preserved in 0.5%~8% Chloramine T Solution

[0026] 1 Materials and methods

[0027] 1.1 Experimental chickens and their feeding

[0028] Qualified Lingnan yellow chicken eggs were purchased from the Animal Husbandry Institute of Guangdong Academy of Agricultural Sciences, hatched until hatched, kept in isolation in flame-sterilized wire cages, fed full-price feed without anticoccidial drugs, and dried at 70°C before use. Roast for 12 hours and drink boiled water. Before the test, the feces were checked for contamination with coccidia oocysts.

[0029] 1.2 Chicken coccidiosis quadrivalent live vaccine

[0030] Developed by Foshan Zhengdian Biotechnology Co., Ltd., containing Eimeria tenella (Eimeriatenella), poisonous Eimeria (E.necatrix), giant Eimeria (E.maxima) and stacked Eimeria Four species of coccidia (E.acervulina) were stored in 0.5%-8% chloramine T solution. In this embodiment, 1% chloramine T s...

example 3

[0038] Example 3 Acute Toxicity Test of Chicken Coccidiosis Quadrivalent Live Vaccine on Mice

[0039] 1 Materials and methods

[0040] 1.1 Test animals

[0041] 40 Kunming mice weighing 18-24 g, half male and half male, were purchased from Zhongshan Medical University. Before the test, they were fed under constant conditions for 7 days, and their health and physiological conditions were good.

[0042] 1.2 Test substance

[0043] Chicken coccidiosis quadrivalent live vaccine, batch number 20040001, developed by Foshan Zhengdian Biotechnology Co., Ltd., contains 0.5% to 8% chloramine T solution.

[0044] 1.3 Test grouping and treatment

[0045] The above 40 Kunming mice were randomly divided into 4 groups, 10 in each group (half male and half male). After fasting for 4 hours, each mouse in groups 1 to 3 was given 10 μl, 50 μl, and 100 μl of chicken coccidiosis quadrivalent live vaccine (shake vigorously before use) by intragastric administration, and group 4 wa...

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Abstract

The invention discloses a coccidian ovisac culture liquid and conservative liquid, which comprises the following steps: adapting this culture liquid and conservative liquid at (0.5-8.0):100 with chloramine T and pure water to form chloramine T solution; separating and purifying fresh coccidian oocyst from ovisac fowl dung; suspending the ovisac sediment in the chloramine T solution at 25-30 deg.c; setting the culture wetness more than 50 percent and ovisac density less than 1000,000 oocyst per ml and culture liquid depth less than 0.7 cm; stirring culture liquid more than three times a day for 24-72 h; stopping cultivating when more than 80 percent oocyst finishes sporing; obtaining oocyst culture liquid; centrifuging condensation; adding chloramine T solution again; stewing at 4-8 deg.c in the fridge. The invention improves the sporing rate of coccidian ovisac culture liquid, which avoids heavy metal material in the chitchen and environmental pollution.

Description

Technical field: [0001] The invention belongs to a veterinary vaccine pathogen culture solution and preservation solution. More precisely, the invention uses chloramine T solution as the culture solution and preservation solution of chicken coccidiosis vaccine coccidia oocysts. Background technique: [0002] Chicken coccidiosis is one of the main diseases that harm the intensive chicken industry, and its prevention and control is still dominated by drugs in recent years. However, with the widespread and continuous use of anticoccidial drugs, the two major problems of drug resistance and drug residues are becoming more and more serious, which has aroused the vigilance of the whole world. With the arrival of green animal husbandry, the use of coccidiosis vaccine immunization to prevent chicken coccidiosis is the general trend. Chicken coccidiosis vaccine has experienced the development process of non-attenuated strain vaccine (coccidia oocyst virulent live vaccine) to attenua...

Claims

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Application Information

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IPC IPC(8): C12N1/10
Inventor 谭志坚翁亚彪
Owner FOSHAN STANDARD BIO TECH
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