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Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell

A technology of dendritic cells and immune tolerance, applied in blood/immune system cells, animal cells, vertebrate cells, etc.

Inactive Publication Date: 2010-05-26
NANKAI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no systematic approach to identify genes controlling immune tolerance in dendritic cells

Method used

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  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell
  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell
  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Preparation of immune tolerant dendritic cells.

[0074] Specific preparation method:

[0075] 1.1. Experimental materials:

[0076] 1). The medium is PRMI1640 medium (Hyclone) containing 10% calf serum (Minhai Biotechnology), and 1% penicillin and 1% streptomycin (Sigma) are added at the same time. The experimental temperature conditions are 37°C. The medium in the following description is this medium.

[0077] 2). Ovarian tumor cells are tumor cells isolated from mouse ovarian tumor tissue; ovarian tumor cells are provided by Dr. Katherine Roby (Carcinogenesis, 21:585-591, 2000). In order to determine whether the induction of tumor-related immune tolerance dendritic cells is through direct cell-cell contact or a factor secreted by the tumor cells themselves, we processed the ovarian cancer cells as follows:

[0078] A. Use 14000 Rad Co 60 Irradiate ovarian cancer cells. Such cells are used to evaluate the effects of direct cell-to-cell contact.

[0079] B. The culture supern...

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Abstract

The provided screen method for RGS1 gene on immune tolerance comprises: preparing human immune tolerance dendritic cell by external culture to increase the expression of RGS1 gene in the dendritic cell; when transfecting siRNA of RGS1 gene or aimed genes with monocyte series, affecting activity of transcription factor and expression; and playing special immunoregulation function to guide immune tolerance. This invention has great application potential.

Description

Technical field [0001] The present invention relates to a method for in vitro induction of immune tolerance dendritic cells to screen the gene RGS1 for immune tolerance-related diseases, specifically the immune tolerance-related disease gene regulator of G protein 1 (hereinafter referred to as RGS1 gene), with new functions of the known gene RGS1 The screening and identification methods found, RGS1 participates in immune tolerance and affects the expression of immune response genes. RGS1 gene has potential clinical application value in the treatment of immune-related diseases such as transplant rejection, tumor, inflammation, and autoimmune diseases. Background technique [0002] In 1975, the famous immunologist Burnet proposed the theory of clonal selection and explained the phenomenon of immune tolerance with the theory of clonal deletion. T and B reactive cell lines in the immature stage are eliminated due to exposure to antigens, which results in immune tolerance. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N5/08C12N5/0784
Inventor 杨荣存刘昱R·卢登W·如适张园张灼寒
Owner NANKAI UNIV
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