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Immunochromatographic assay test paper for diagnosing psittacosis chlamydia infection and preparation method thereof

A technology of chlamydia psittaci and immunochromatographic test paper, which is applied in the field of immunochromatographic test paper and its preparation, can solve the problems of long time required and difficult on-site use, etc., and achieve the effect of strong specificity, easy operation and good stability

Inactive Publication Date: 2012-05-09
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The time required is longer than the immunochromatographic test strip method and it is not easy to use in the field

Method used

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  • Immunochromatographic assay test paper for diagnosing psittacosis chlamydia infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of two kinds of immunochromatographic test papers that detect Chlamydia psittaci infection

[0042] like figure 1 As shown, the immunochromatographic test paper for detecting Chlamydia psittaci infection is composed of four parts: absorbent pad 4, nitrocellulose membrane (NC membrane) 3, gold standard pad 2 and glass fiber membrane sample pad 1, which are closely connected in series; nitrocellulose membrane 3 The upper package is covered with detection line 5 and quality control line 6.

[0043] 1. Preparation of Chlamydia psittaci MOMP antigen

[0044] The Chlamydia psittaci MOMP antigen is a protein having the amino acid residue sequence of Sequence 2 in the sequence listing of the invention patent with the patent number 02146790.0. Specifically, it can be prepared as follows:

[0045] Design primers to amplify the nucleotide sequence fragment (the coding gene of the main outer membrane protein of Chlamydia psittaci) in the sequence li...

Embodiment 2

[0063] Embodiment 2, the effect experiment of detection clinical serum sample

[0064] The immunochromatographic test paper that detects mammalian Chlamydia psittaci infection with the preparation of embodiment 1 detects 93 parts of chlamydia psittaci infecting bovine serum, and the immunochromatographic test paper that detects chicken chlamydia psittaci infection detects 250 parts of chlamydia psittaci infected chicken serum, will The bovine or chicken serum sample infected by Chlamydia psittaci was diluted with normal saline at 1:40, with normal bovine serum or chicken serum as the control, also diluted with normal saline at 1:40, and the serum titer above 1:40 was positive Diagnostic criteria.

[0065] The results of bovine serum samples detected by the immunochromatographic test paper for detecting Chlamydia psittaci infection showed that among 93 chlamydia-infected bovine sera, 69 were positive and 24 were negative. 50 normal cattle (control) were all negative by immunoc...

Embodiment 3

[0067] Embodiment 3, compare detection effect with ELISA method, IHA method

[0068] Detect 22 parts of bovine serums with the immunochromatographic test paper detection (ICA detection) of the detection mammalian chlamydia psittaci infection prepared in embodiment 1, detect 22 parts of chicken serums with the immunochromatographic test paper detection (ICA detection) of chicken chlamydia psittaci infection . At the same time, the Chlamydia psittaci MOMP antigen prepared in Example 1 was used for detection by ELISA method. These sera are from cows (or sick chickens) and normal cows (or normal chickens) with chlamydia psittaci disease in the same area, and they are used for the specificity evaluation of immunochromatographic test paper for detecting chlamydia psittaci infection, with good comparability .

[0069] Detecting result shows 22 parts of bovine sera (positive for Chlamydia psittaci through ELISA and IHA method inspection), 19 parts of positives are tested with ICA ki...

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Abstract

The disclosed immune chromatography test paper to detect chlamydia psittaci comprises: in series, a sample pad, a gold marked pad contained colloid gold probe marked by staphylococcus aureus protein A or IgG, a fiber film with separated detection line made of main out film protein of chlamydia psittaci and quality-control line as antibody fit to combine with former protein A or IgG, and the absorbent pad. Compared with IHA method, this invention has strong specificity and well sensitivity, and more fit to clinic.

Description

technical field [0001] The invention relates to an immunochromatographic test paper for detecting chlamydia psittaci infection and a preparation method thereof. Background technique [0002] Chlamydia psittaci (Cps) can infect various birds, poultry and domestic animals, causing various diseases. The disease not only causes economic loss in animal husbandry and poultry industry, but also seriously threatens human health. The main hosts of Cps are birds and birds, and mammals and humans can also be infected after close contact. Farming countries such as Australia and New Zealand list Cps as one of the most important pathogenic microorganisms that must be controlled. Due to its strong lethality and incapacity, Cps is a classic biological warfare agent. [0003] Cps can cause psittacosis, fever, headache, cough, interstitial pneumonia, and myocarditis in the crowd; it can cause arthritis, urethritis, and conjunctivitis syndrome in humans; 200 kinds of animals can be infected...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/544G01N21/78G01N33/52G01N33/532
Inventor 端青朱虹宋立华张美玲檀华何君
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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