A method for preparing hirudin by making leech bloodsucker as raw material and application thereof
A blood-sucking leech and hirudin technology is applied in the fields of medical raw materials derived from leech/helminth, cardiovascular system diseases, metabolic diseases, etc., which can solve the problems of expensive equipment and high drying cost, and achieves low production cost, and is convenient to carry and take. , the effect of stable quality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0034] Take 10 kilograms of the live body of Hirudo phenanthae, rinse it with clean water, add 100 kilograms of normal saline, and homogenize it repeatedly in a colloid mill for 3 times to obtain the Hirudo phenanthae slurry; filter the Hirudo phenanthae slurry with an 80-mesh sieve, Obtain 104.3 kilograms of filtrate after removing the filter residue, because there are still some undigested blood and its own protein in the living body of Hirudo phenanthae; Add 95 kilograms of common tapioca starch and 5 kilograms of lactose respectively in the above-mentioned filtrate, stir immediately Obtain 204.3 kilograms of suspension liquid; Put above-mentioned suspension liquid in the spray drier and carry out spray drying, its condition is 175 ℃ of inlet temperature, 78 ℃ of outlet temperature, obtain altogether 101.5 kilograms of dry powders, because there are still some Some undigested blood and its own protein; the content of natural hirudin in the above-mentioned powder is 61ATU / gra...
Embodiment 2
[0036] Get 30 kilograms of leech cadavers preserved in freezing, add 210 kilograms of drinking water after it dissolves, and obtain leech slurry after repeated homogenization 4 times in a colloid mill; Filter, obtain 221.7 kilograms of Japanese medicine leech filtrate after removing filter residue, because of still some undigested blood in the Japanese medicine leech corpse; Add 50 kilograms of cornstarch in above-mentioned filtrate, promptly obtain suspension 271.7 kilograms after stirring; The suspension was spray-dried in a spray dryer under the conditions that the inlet temperature was 185°C and the outlet temperature was 81°C, and a total of 52.9 kg of dry powder was obtained, because there were still some undigested blood and its own blood in the Japanese medical leech corpse. Protein; the content of natural hirudin in the above-mentioned powder is 436ATU / gram through the direct titration method of Markwardt thrombin detection.
Embodiment 3
[0038]Take 20 kilograms of the dead body of Hirudo phenanthae that is frozen and preserved, add 20 kilograms of normal saline after it dissolves, and obtain the Hirudo phenatus slurry after repeated homogenization 5 times in the colloid mill; Filtration, after removing the filter residue, 26.6 kilograms of filtrate was obtained, because there was still some undigested blood in the corpse of Hirudo phenanthae; 3 kilograms of common tapioca starch and 0.4 kilogram of mannitol were added respectively to the above-mentioned filtrate, and the suspension was obtained after stirring evenly. 30 kg of turbid liquid; the above suspension is placed in a spray dryer and spray-dried under the condition that the inlet temperature is 168°C and the outlet temperature is 68°C, and a total of 4.2 kg of dry powder is obtained. Digested blood and its own protein; the content of natural hirudin in the dry powder detected by Markwardt thrombin direct titration method is 3809.5ATU / gram.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com