Reovirus vaccine and its preparing method

A preparation technology for reovirus and vaccine, which is applied in the fields of virus vaccine and preparation, reovirus and vaccine preparation, can solve the problems of unsatisfactory prevention and treatment and high price, and achieve the effect of making up for the vacancy of vaccines

Active Publication Date: 2006-12-27
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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Problems solved by technology

The use of foreign vaccines is not very ideal for the preve...

Method used

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Embodiment Construction

[0014] The vaccine of the present invention is aimed at native reovirus RS13 strain, the virus has no capsule, is icosahedron symmetric, and its genome is composed of 10 double-stranded RNA segments. It is insensitive to ether, slightly sensitive to chloroform, and sensitive to alkali. The virus can be killed by 3% sodium hydroxide for 1 hour, and the virus has no hemagglutination properties.

[0015] The virus is easy to grow in chicken embryos, and the yolk sac can be used for inoculation when it is isolated for the first time. Generally, the chicken embryos die 3 to 5 days after inoculation, and the chicken embryos are purple due to extensive subcutaneous hemorrhage. When the chorioallantoic membrane is inoculated, it usually dies within 7 to 8 days. The chorioallantoic membrane has slightly raised and scattered white lesions, and the chicken embryo is mildly stunted.

[0016] The virus can also reproduce on chicken embryo fibroblasts, but it needs to adapt, that is, the ce...

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Abstract

A reovirus vaccine and its method for preparation belong to the technical sphere of druggist sundries and are used to solve the indigenous fowl reovirus infection vaccine problem. The separated reovirus is of CGMCC No.1713 as its preserved number and has been preserved in China microbial bacterial preservation management committee common microorganism center on May 16th, 2006. The said vaccine is prepared by steps including virus isolation from organ of diseased chicken, serum check, virus breeding and generation and cryodesiccation. The invention separates the virus from the diseased chicken with ARV infection of indigenous fowls, applies them in immune chicken, and produces immune response within 1 week and produces strong immunity within 2 weeks. The immune time is 2-2.5 months, and the detoxicating protecting rate during immune period is 94.8%-98.8%, which is explicitly higher than import vaccine protecting rate and can make up for the domestic defect of vaccine vacancy in reovirus affection.

Description

technical field [0001] The invention relates to a virus vaccine and a preparation method thereof, in particular to a reovirus and a preparation method of the vaccine, and belongs to the technical field of medical products. Background technique [0002] Avian reovirus (Avianreovirus, ARV) is one of the main pathogens of chicken viral arthritis and tenosynovitis, belonging to Reoviridae, a member of Reovirus genus. Since Fahey and Grawly (1954) first isolated (ARV) from the respiratory tract of chickens suffering from chronic respiratory diseases, many ARV isolates have been reported successively. With the development of research, the relationship between ARV and other diseases has also attracted people's attention. It has been found that in addition to viral arthritis and tenosynovitis, ARV plays a certain role in the occurrence of diseases such as respiratory diseases, intestinal diseases, heart and liver lesions, blue wing disease, broiler dwarf syndrome, and temporary dig...

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Application Information

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IPC IPC(8): C12N7/00A61K39/12A61P31/12
Inventor 厉从志冯秀丽
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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