Standard chip for biological PCR micro path fluorescent detection and its preparing method

A fluorescence detection and microchannel technology, applied in the field of biology and medical detection, can solve the problems of small signal emission point and weak detected fluorescence spectral signal, and achieve the effect of solving work calibration

Inactive Publication Date: 2007-01-24
BEIJING UNIV OF TECH
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Problems solved by technology

There are some differences from the fluorescence spectrum detection technology in the field of chemical analysis: First, the amount of the detected object is small and the signal emission point is small, resulting in a weak detected fluorescence spectrum signal, and a micro-channe

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  • Standard chip for biological PCR micro path fluorescent detection and its preparing method
  • Standard chip for biological PCR micro path fluorescent detection and its preparing method
  • Standard chip for biological PCR micro path fluorescent detection and its preparing method

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Embodiment Construction

[0022] Combine below Figure 1 ~ Figure 3 Examples of the present invention will be described. The chip mainly includes an upper piece of PMMA organic glass 3 and a lower piece of PMMA organic glass 4, and the thickness of the upper piece of organic glass 3 and the lower piece of organic glass 4 is 1mm. Wherein, six independent analog microchannels 2 are arranged on the bottom plexiglass 4, micropores 1 are respectively processed on the two ends of the corresponding simulation microchannel on the upper plexiglass 3 and the micropores 1 are sealed, and the upper plexiglass 3 and the lower piece of plexiglass 4 are permanently sealed by thermocompression bonding; fluorescent substances with increasing fluorescence intensity are sequentially injected into the six simulated microchannels 2, and the selected fluorescence intensity can reflect the specificity of this biological PCR fluorescent reagent. The change trend of the copy number amplification curve of the gene.

[0023] T...

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Abstract

The chip includes upper piece of organic glass, and lower piece of organic glass. N pieces of independent simulation micro channel are setup on the said lower piece of organic glass. Being setup on upper piece of organic glass, micropores are corresponding to two ends of the said micro channels, and micropores are sealed. Using hot pressing linkage seal upper piece of organic glass and lower piece of organic glass permanently. Fluorescent materials with incremental fluorescence intensities are injected to N pieces of independent simulation micro channel in sequence. The selected fluorescence intensities can reflect changing trend of amplification curve of number of replications for characteristic gene of the biologic PCR fluorescent reagent. The invention solves issue of working calibration of detection device for PCR fluorescence spectrum.

Description

technical field [0001] The invention relates to a measurement comparison device of a PCR (polymerase chain reaction) fluorescence microchannel spectrum detection system for a biochemical micro-full analysis system chip, which is mainly used for the calibration of a microchannel fluorescence spectrum detection device and belongs to the field of biology and medical detection . Background technique [0002] As the concept of Miniaturized Total Analysis Systems (μ-TAS) was first proposed in the early 1990s, in recent years, various biological analysis and chemical analysis chips (including: micro Fluidic bio-PCR chip) research is developing rapidly, and micro-analysis system technology has become the most important research and development direction of innovative analytical testing instruments and technologies, and has attracted the attention of countries all over the world. [0003] The so-called micro-total analysis system (μ-TAS) is an analysis laboratory system that realize...

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Application Information

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IPC IPC(8): G01N33/48G01N21/64
Inventor 吴坚古冬冬
Owner BEIJING UNIV OF TECH
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