Insecticidal crystalline gene cry7Bal of Bacillus thuringiensis

A technology of Bacillus thuringiensis, insecticidal crystal protein, applied in genetic engineering, plant genetic improvement, biocide and other directions
CN1952151AInactive Publication Date: 2007-04-25HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2007-04-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses the separation, clone and application of insecticidal crystal protein gene from Bacillus thuringiensis. In this invention a novel insecticidal crystal protein cry7Ba1 is separated from Bacillus thuringiensis Central China species YBt-978 strains. The genes code a novel insecticidal crystal protein Cry7Ba1 with insecticidal activity to Lepidoptera insects. Cry7Ba1 protein is consisted by 1,154 amino acid, and its homology with the existing published proteins is no more than 80% and the half of amino acid sequence near the protein N-terminal has no more than 50% homology with the existing published proteins. The invention also discloses the gene sequence of this novel insecticidal crystal protein and its coding protein Cry7Ba1 expressed by microorganism transformed by appropriate expression vector, qualifying it with lepidoptera insecticidal activity.
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Description

technical field

[0001] The invention belongs to the technical field of microbial genetic engineering. Specifically, it relates to the isolation and cloning of an insecticidal crystal protein gene from Bacillus thuringiensis, which is related to the genetic engineering of biological pesticides. Background technique

[0002] Bacillus thuringiensis is a rod-shaped, Gram-positive bacteria that can form endogenous spores and widely exists in various ecological environments. In the budding stage, it can form parasporal crystals composed of insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs) that are specifically toxic to insects. More than 500 kinds of insects in 10 orders of Insecta and some harmful species in protozoa, nematodes and flatworms also have specific biological activities (Schnepf H E, Crickmore N, Rie J V, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean D H. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev, ...

Claims

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