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Aminoacylase 1 and application for examining liver cancer by antibody of same

An acylated amino acid and hydrolase technology, which is applied in the application field of acylated amino acid and its antibody for detecting liver cancer, can solve the problems such as the correlation between acylated amino acid hydrolase 1 and hepatocellular carcinoma, etc., which is beneficial to popularization and application, stable Good and specific effect

Inactive Publication Date: 2007-05-23
上海中科新生命生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But so far, there is no report about the correlation between acylamino acid hydrolase 1 and hepatocellular carcinoma in the prior art

Method used

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  • Aminoacylase 1 and application for examining liver cancer by antibody of same
  • Aminoacylase 1 and application for examining liver cancer by antibody of same
  • Aminoacylase 1 and application for examining liver cancer by antibody of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, Preparation of protein samples of hepatocellular carcinoma tissue and paracancerous tissue

[0029] Urea, 3-[(3-cholamidopropyl)-diethylammonium]-1-propanesulfonic acid (CHAPS), phenylmethylsulfonyl fluoride (PMSF), dithiothreitol used in this example (DTT) were purchased from Sigma Company.

[0030] In this example, protein samples of cancer tissue and paracancerous tissue of hepatocellular carcinoma were prepared by nonenzymatic sample preparation (NESP), as follows:

[0031]The surgically excised fresh tissue block is quickly placed on ice and quickly cut into several small pieces that are visible to the naked eye without areas of necrosis. Use pre-cooled glutamine-free RPMI1640 medium (5% fetal bovine serum, 0.2mMPMSF, 1mM EDTA, benzisoxazole penicillin 25mg / mL, gentamicin 50mg / mL, penicillin 100U / mL, strepto Amphotericin 100mg / mL, Amphotericin B 0.25mg / mL, Nystatin 50U / mL) After washing the tissue pieces several times, they were quickly ground into cel...

Embodiment 2

[0035] Example 2, Screening of Differentially Expressed Proteins 1

[0036] Urea, 3-[(3-cholamidopropyl)-diethylammonium]-1-propanesulfonic acid (CHAPS), sodium dodecylsulfonate (SDS), dithiothreitol used in this example (DTT) was purchased from Sigma; iodoacetamide (IAA), acrylamide, N, N-methylenebisacrylamide, etc. were purchased from Fluka.

[0037] Ammonium persulfate (AP), Tri-n-butylphosphat (TBP), PDQuest software, etc. are Bio-Rad products.

[0038] Bruker Reflex III MALDI-TOF mass spectrometer was purchased from Karlsruhe (Germany).

[0039] Non-linear immobilized pH gradient prefabricated strips (IPG dry strips, pH3-10NL, 130×3×0.5mm), IPG buffer, IPGphore isoelectric focusing system (Amersham Pharmacia Biotech), Amersham Pharmacia Ettan Dalt II systems, etc. Product of Amersham Bioscience.

[0040] Take the first 10 pairs (f31, f32, f33, f39, 327, 328, 415, 418, 422 and 317 in Table 1) of the 16 pairs of hepatocellular carcinoma cancer tissues and paracancerous ...

Embodiment 3

[0060] Example 3, Screening of Differentially Expressed Proteins II

[0061] Urea, 3-[(3-cholamidopropyl)-diethylammonium]-1-propanesulfonic acid (CHAPS), and dithiothreitol (DTT) used in this example were purchased from Sigma; iodoacetamide (IAA), acrylamide, N, N-methylenebisacrylamide, etc. were purchased from Fluka.

[0062] Ammonium persulfate (AP), TEMED, Tri-n-butylphosphat (TBP), Sypro Ruby fluorescent dyes, etc. are Bio-Rad products.

[0063] LCQTM Deca XP system and ProteomeXTM Workstation were purchased from ThermoFinnigan Company.

[0064] Cy2, Cy3, Cy5 fluorescent dyes, non-linear immobilized pH gradient precast strips (IPG dry strips, pH3-10NL, 130×3×0.5mm), IPG buffer, IPGphore isoelectric focusing system, AmershamPharmacia Ettan Dalt II system , Typhoon scanner, automatic glue cutter, DeCyder TM Analysis software, etc. are all products of Amersham Bioscience.

[0065]Get 10 pairs (f31, f32, f33, f39, 327, 328, 415, 418, 422 and 317 in Table 1) in the 16 pai...

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Abstract

According to the acylized amino acid hydrolase enzyme 1 having different expression in hepatocellular carcinoma tissues and carcinoma surrounding tissues, the invention uses it for the detection of hepatocellular carcinoma protein molecular marker, and then using the antibody of acylized amino acid hydrolase enzyme 1, to prepare the solution or reagent box for the detection of hepatocellular carcinoma. The method has high detection accuracy rate, strong specificity, simple operation, and can be widely used in clinical diagnosis.

Description

technical field [0001] The present invention relates to an application of acylated amino acid hydrolase 1 and its antibody, in particular to the application of acylated amino acid and its antibody to detect liver cancer. Background technique [0002] Liver cancer is a disease that seriously endangers human beings. The incidence of liver cancer in western developed countries is low, and the basic research on liver cancer in the world is still relatively weak. However, my country is a country with a high incidence of liver cancer, and the morbidity and mortality are on the rise, and the age of onset is younger. Medical expenses have increased greatly, and liver cancer has become the number one enemy that seriously endangers the safety of people's life and property in our country, and is an important factor affecting social and economic development. It is of strategic significance to intensify the basic research of liver cancer in my country, and to separate and identify new Liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/574G01N33/577C12Q1/00
Inventor 李辰周晓
Owner 上海中科新生命生物科技有限公司
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