Pig's epidermal growth factor gene and its application

An epidermal growth factor and gene technology, applied in the field of genetic engineering, can solve the problems of high price and difficult to meet production needs, and achieve the effects of low production cost and simple purification
CN1974767AInactive Publication Date: 2007-06-06SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2007-06-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides one kind of pig's epidermal growth factor gene with the nucleotide sequence shown in SEQ ID No. 1 of the sequence list. The present invention provides also the gene engineering bacterium C32 containing 14 copies of the gene, and the gene engineering bacterium has high pEGF expressing amount, simple purifying process and low production cost. The present invention makes it possible to produce pig's epidermal growth factor gene in industrial scale.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a porcine epidermal growth factor gene, and also relates to an expression vector constructed by the gene and a transformant thereof. Background technique

[0002] Epidermal growth factor is a protein that Cohen (1962) isolated from the submandibular gland of male mice in the 1960s, which can make the eyelids of newborn mice open early and the teeth erupt early. Later, human epidermal growth factor was isolated from human urine in 1975. Human and mouse epidermal growth factor can stimulate the proliferation of epidermal and endothelial cells, and can be used to treat burns, corneal trauma, gastrointestinal ulcers, etc. In 1991, the porcine epidermal growth factor gene was cloned and expressed in yeast. It was found that the expressed porcine epidermal growth factor could promote the DNA synthesis of Kunming mouse 3T3 cells (Pascall JC, Jones DS, Doel SM, Clements JM, Hunter M, ...

Claims

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