Fermentative production of fine chemicals

A technology for fermentation broth and microbial fermentation, applied in the fields of fermentation, chemical recovery, biochemical equipment and methods, and can solve problems such as not being widely used, complex, and not optimized.

Inactive Publication Date: 2007-06-13
BASF SE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Thus, although it is technically possible to use cassava as a starch feedstock in methods correspo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0241] Example 1

[0242] a) Enzymatic starch liquefaction and starch saccharification

[0243] 500 g of dry ground corn flour was suspended in 750 ml of water and finely ground again in a blender. The suspension was divided into 4 sample numbers 1 to 4, each of which was treated with about 3 g of thermostable α-amylase (sample numbers 1 and 2: Termamyl L; sample numbers 3 and 4: Spezyme). Sample numbers 2 and 4 were then treated with about 7 g / l glucoamylase (sample number 2: Dextrozyme GA; sample number 4: Optidex). This produces a pale yellow viscous sample, each of which has a solid content separated by centrifugation, in which a layer of hydrophobic solid floats above the clear liquid phase.

[0244] The clear supernatant of the resulting sample (concentrated form and 10-fold dilution) was analyzed by HPLC, ignoring or considering centrifugal sedimentation. When considering the sediment, a 50% dry matter content of the sediment is assumed. The results based on the starting sa...

Example Embodiment

[0265] Example 2: Fermentation

[0266] Using the corn meal hydrolysate obtained as described in Example II.1, using the strain ATCC13032 lysC described in III fbr , Similar to Example 1b) for fermentation. The cells were incubated on sterile CM agar (constitute: see Table 4; 121°C for 20 minutes) at 30°C for 48 hours. The cells were then scraped from the plate and resuspended in saline. Inoculate 25ml of medium 1 or 2 in a 250ml Erlenmeyer flask with this amount of cell suspension (see Table 5) so that the optical density reaches OD at 610nm 600 The value is 1. The samples were then incubated for 48 hours at 200 rpm in a humid shaker (relative atmospheric humidity of 85%) at 30°C. The lysine content in the medium was determined by HPLC. In all cases, approximately the same amount of lysine was produced.

Example Embodiment

[0267] Example 3

[0268] Use Corynebacterium glutamicum (ATCC13032 lysC fbr In the shake flask experiment of ), two corn flour hydrolysates (bottle 1+2) obtained according to Example II.3a were used. In addition, the rye flour hydrolysate (bottle 5+6) and the wheat flour hydrolysate (bottle 3+4) prepared similarly in Example II.3 were used at the same time.

[0269] 3.1) Preparation of inoculum

[0270] The cells were streaked on sterile CM+CaAc agar (composition: see Table 7; 121°C for 20 minutes) and then incubated at 30°C for 48 hours, then inoculated to a new plate and incubated at 30°C overnight. The cells were then scraped from the plate and resuspended in saline. Inoculate 23ml of medium in a 250ml Erlenmeyer flask equipped with two baffles (see Table 8) with this amount of cell suspension so that the optical density reaches OD at 610nm 610 The value is 0.5.

[0271] Table 7: Composition of CM+CaAc agar plate

[0272] Concentration

[0273] 20.0g / l ...

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PUM

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Abstract

The invention relates to a method for the production of at least one microbial metabolic product having at least 3 C atoms or at least 2 C atoms and at least one N atom by means of sugar-based microbial fermentation. The inventive method involves the following steps: a) producing a sugar-containing liquid medium having a monosaccharide content of more than 20 weight percent from a starch source, wherein the sugar-containing liquid medium also comprises nonstarch-containing solid components of the starch source; b) fermenting the sugar-containing liquid medium for the production of the metabolic product(s) and c) separating or isolating at least one metabolic product from the fermentation broth, wherein the microorganism strain producing the desired metabolic product(s) is cultivated with the sugar-containing liquid medium, which is obtained by: a1) milling the starch source and a2) liquefying the ground material in an aqueous fluid in the presence of at least one starch liquefying enzyme and subsequently saccharifyng the resulting liquid using at least one saccharifying enzyme, wherein at least part of the ground material is liquefied into an aqueous liquid by continuous or discontinuous addition.

Description

Invention field [0001] The invention relates to the production of fine chemicals by grinding, liquefying and fermenting saccharified starch raw materials, and the use of the produced sugar solution as a fermentation medium. Background technique [0002] A fermentation method for producing fine chemicals (such as amino acids, vitamins and carotenoids) by microorganisms is known. Different carbon raw materials are used according to the conditions of various methods. They range from pure sucrose produced from beet and sugar cane molasses to the well-known high-quality molasses (converted cane molasses) to glucose derived from starch hydrolysates. In addition, acetic acid and ethanol are mentioned as co-substrates that can be used to produce L-lysine in industrial-scale biotechnology (Pfefferle et al., Biotechnological Manufacture of Lysine, Advances in Biochemical Engineering / Biotechnology, Volume 79 (2003), 59-112) . [0003] Based on the above carbon raw materials, a variety of me...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12P13/24C12P13/02C12P19/02C12P13/08C12P19/14C12P13/10C12P19/20C12P13/12C12P25/00C12P13/14C12N15/54C12P1/00C12P13/00
CPCC12P13/12C12P13/10C12P19/02C12P19/14C12P13/08C12P7/46C12P13/24C12P19/20C12P25/00C12P13/14C12P13/02Y02P20/582C12P13/00C12N15/52
Inventor M·蓬佩尤斯S·弗里尔M·洛沙德伊特O·策尔德尔M·博伊
Owner BASF SE
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