DNA carrier, method of producing the same and collection system using the same

A carrier and porous matrix technology, applied in the field of DNA carriers, can solve the problems of insufficient DNA capacity and small DNA exposure area.

Inactive Publication Date: 2007-06-20
CANON KK +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these methods, there are still problems that the DNA exposure area is small and the DNA capacity is not fully displayed

Method used

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  • DNA carrier, method of producing the same and collection system using the same
  • DNA carrier, method of producing the same and collection system using the same
  • DNA carrier, method of producing the same and collection system using the same

Examples

Experimental program
Comparison scheme
Effect test

Synthetic example 1

[0053] 40 g of N,N-dimethylaminopropyltrimethoxysilane (207.34→138.34) was added dropwise to 200 g of distilled water and hydrolyzed at room temperature for 3 days. The resulting oligomer solution was concentrated at 60°C with an evaporator. Thereafter, 80 g of distilled water was added thereto to obtain about 180 g of a basic functional group-bearing siloxane solution (N1) with a solid content of 14.8%.

Synthetic example 2

[0055] 40 g of N-methylaminopropyltrimethoxysilane (193.32→124.32) was added dropwise to 200 g of distilled water and hydrolyzed at room temperature for 3 days. The resulting oligomer solution was concentrated at 60°C with an evaporator. Thereafter, 70 g of distilled water was added thereto to obtain 170 g of a basic functional group-bearing siloxane solution (N2) having a solid concentration of about 15.1%.

Synthetic example 3

[0057] 40 grams of organosilica compounds (262.32 → 193.32) represented by the following formula:

[0058] Chemical formula (7)

[0059]

[0060] 200 g of distilled water was added dropwise and hydrolyzed at room temperature for 3 days.

[0061] The resulting oligomer solution was concentrated at 60°C with an evaporator. Thereafter, 70 g of distilled water was added thereto to obtain about 200 g of a siloxane solution (N3) having a basic functional group with a solid content concentration of 14.7%.

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Abstract

Provided are a DNA carrier where DNA is firmly held in a substrate, which can reduce the elution of DNA into water and can take full advantage of the capability of DNA to selectively and specifically collect a substance; and a method of producing the DNA carrier. Also provided is a collection system using DNA, which can be used in the high-accuracy detection of a particular substance and in an environmental cleanup capable of efficiently removing a substance, in which the DNA carrier is used to collect the substance contained in air or water by taking full advantage of the capability of DNA to selectively and specifically collect the substance. The DNA carrier is one where DNA is held in a porous matrix containing polyorganosiloxane with a basic functional group and particles. Preferably, the polyorganosiloxane with a basic functional group contains a hydrolysis condensate of one or more of particular silane compounds with an amino group.

Description

technical field [0001] The present invention relates to DNA vectors. In particular, the present invention relates to a DNA carrier that firmly immobilizes DNA in a porous matrix, which reduces the elution of DNA into water even when in contact with water, and maintains the selective recognition ability of DNA and the incorporation of specific substances into DNA double Capability in a helix; also relates to a method of making the DNA vector and a collection system using the DNA. Background technique [0002] DNA (deoxyribonucleic acid) is responsible for genetic information in a living body and is one of the most important substances for life phenomena. DNA has the ability to recognize molecules with great precision because single-stranded DNA forms many base pairs with complementary single-stranded DNA. DNA enables the selective and specific intercalation (incorporation) of aromatic compounds having a planer chemical structure into its double helix, and thus is regarded a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L83/08C02F1/28B01J20/24C07H21/04
CPCC08L83/08C02F1/285B01J2220/4856B01J20/24B01J20/28028B01J20/28004B01J20/28026
Inventor 张祖依榊原悌互小谷佳范松村和之西则雄
Owner CANON KK
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