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Method and composition for treatment of headache using magnesium

a technology of magnesium and composition, applied in the field of magnesium-based composition for headache treatment, can solve the problems of inability to quickly make such determinations, inconvenient use, and inability to precisely determine the exact proportion of these fractions, so as to prevent magnesium imbalances and prevent magnesium-associated disease states

Inactive Publication Date: 2001-08-02
ALTURA BURTON M +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The exact proportion of these fractions has been extremely difficult to determine precisely, and, moreover, there is no way to rapidly make such determinations.
While this procedure is capable of measuring free Mg.sup.2+, it is fraught with a multiplicity of problems (need to control pH, need to control Filter composition, time-consuming, inability to access whole blood Mg.sup.2+, need for centrifugation of blood).
Moreover, determinations can not be done on less than 1.0 ml of blood.
Accelerated atherosclerotic heart disease is a leading cause of death in the long-term (>10 year) renal transplant recipient.
Although major advances have been made in unraveling the etiologies of numerous disease states, the precise cause(s) of migraine headaches remains elusive.
This spreading depression causes an attenuation of blood flow to the affected area of the brain, resulting in local hypoxia and possibly the neurological phenomenon termed the aura of migraine.

Method used

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  • Method and composition for treatment of headache using magnesium
  • Method and composition for treatment of headache using magnesium
  • Method and composition for treatment of headache using magnesium

Examples

Experimental program
Comparison scheme
Effect test

example ii

Sample Processing

[0061] To process clotted blood or plasma (heparinized blood), the samples were maintained under conditions that minimize or prevent exchange of gases between atmospheric air and the biological sample, preferably anaerobic conditions in tubes sealed with rubber stoppers. Parafilm or plastic and glass tops cannot be utilized as this allows for air to enter the sample. If samples (i.e., whole blood, serum or plasma) were analyzed (or frozen) more than 30 min after blood draw for processing of serum or plasma, the sealed tubes were placed in a standard clinical or laboratory centrifuge and centrifuged at 3,000-4,000 rpm for 15-20 minutes. After this time, the sera or plasma was carefully removed from the packed cells by inserting a sterilized needle attached to either a plastic syringe (for sera) or a lightly heparinized (<75 u / ml, more preferably <50 u / ml, most preferably <20 u / ml) glass syringe (for plasma) or a similar device.

example iii

Sample Storage

[0062] The anaerobically maintained samples were either processed with the ISE within six hours or the plasma or serum carefully expelled into a tube with the air evacuated or other tube or syringe substantially free of atmospheric air, especially O.sub.2, most preferably anaerobic, with or without heparin, and frozen at -10.degree. C. Unlike ionized Ca.sup.2+, ionized Mg.sup.2+ levels were stable during numerous subsequent freeze-thaw procedures, provided the bloods were drawn and processed under anaerobic conditions as described above (see FIG. 2, Table 1). Under conditions where parafilm coverings were used, pH and pCO.sub.2 changes occurred causing erratic and erroneous ionized Mg.sup.2+ values.

1TABLE 1 Influence of Freeze-Thawing with Parafilm Covering on Plasma pH and Ionized Free Mg.sup.2+ Obtained with an Ion Selective Electrode Day of Examination Parameter 1 2 3 12 pH 7.435 7.685 7.897 8.26 .+-. .+-. .+-. .+-. 0.010 0.044 0.056 0.070 Mg.sup.2+(mM) 0.64 0.65 0....

example iv

Sample Preparation for Precision, Specificity and Interferant Studies

[0064] Male and female subjects that had no electrolyte abnormalities, ages ranging from 19-83 years, were used for the reference range study.

[0065] Whole blood and plasma samples were collected using a needle attached to heparinized Vacutainer tubes; serum from-red top Vacutainer tubes. All blood samples were collected and maintained under anaerobic conditions and processed within 1-2 hours of collection. Ultrafilterable Mg was obtained using an Amicon micropartition system (3,000 MW cutoff) after centrifugation of the plasma or serum at 1,500 to 2,000 RCF (g) for 20 minutes. A 3,000 MW cutoff was utilized in order to retain small molecular wt peptides. However, when normal sera from six volunteers were processed using a 30,000 MW cutoff virtually identical results were obtained.

[0066] Precision (within run, day-to-day) was determined on three levels of aqueous control solutions obtained from NOVA Biomedical conta...

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Abstract

A method for monitoring ionized Mg2+ concentrations and ionized Ca2+:Mg2+ ratios in a patient, useful in diagnosing and prognosing disease states including headache, cardiopulmonary bypass surgery, hypertension, abnormal pregnancy, head trauma, and fetal abnormalities is provided as well as a composition and a method of treating a patient with ionized Mg2+ or ionized Ca2+ and Mg2+. A method of treating headache is provided.

Description

[0001] This application is a continuation-in-part of pending U.S. Ser. No. 07 / 864,646, filed Apr. 7, 1992 which is a continuation-in-part of pending U.S. Ser. No. 07 / 681,940 filed Apr. 8, 1991.[0002] Magnesium (Mg) is the second most abundant cation in the body [Altura, B. M. et al., Drugs 28 (Suppl.I):120-142, 1984]. It is cofactor for more than 325 cellular enzymes involved in cellular energy production and storage, protein synthesis DNA and RNA synthesis, cell growth and reproduction, adenylate cyclase synthesis, maintenance of cellular electrolyte composition, and stabilization of mitochondrial membranes [Altura, B. M. et al, Drugs 28 (Suppl.I): 120-142, 1984; Wacker, W. E. C. Magnesium and Man, Harvard Univ. Press, Cambridge, 1980]. As a consequence of these biochemical activities, Mg plays a pivotal role in control of neuronal activity, cardiac excitability, neuromuscular transmission, muscular contraction, and vasomotor tone [Altura, B. M. et al., Drugs 28 (Suppl.I):120-142, ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23L1/304A61K31/28A61K33/06A61K33/42G01N33/487G01N33/50G01N33/84
CPCA61K31/28A61K33/06A61K33/42G01N33/84G01N2800/04G01N2800/2807A61K2300/00Y10S426/801Y10T436/109163Y10T436/106664Y10T436/255
Inventor ALTURA, BURTON M.ALTURA, BELLA T.MAUSKOP, ALEXANDER
Owner ALTURA BURTON M
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