Flow cytometer for differentiating small particles in suspension
a flow cytometer and suspension technology, applied in the direction of optical radiation measurement, instruments, spectrometry/spectrophotometry/monochromators, etc., can solve the problems of truncating a portion of the beam divergence, affecting the performance of flow cytometers, and affecting the ability to detect side-scattered radiation
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[0016] Referring now to the drawings, FIG. 1 schematically illustrates a conventional laser diode LD of the type that emits a diverging laser beam LB having an elliptical cross-section that expands in size in accordance with two different angles of divergence, θ1 and θ2. As illustrated, these angles are measured in mutually perpendicular planes, and one angle, in this case angle θ1, is usually substantially larger than the other. As is characteristic of laser diodes, the emitted laser beam LB will be plane-polarized in a plane P that is parallel to the major axis A′ of the expanding elliptical cross-section of the beam. The minor axis A″ of the elliptical cross-section is, of course, perpendicular to the major axis A′.
[0017] In FIG. 2, the laser diode LD of FIG. 1 is shown as being embodied in a flow cytometer of the earlier type described above. A particle sample PS containing particles to be analyzed, e.g., blood cells, is introduced by a nozzle 7 into an optically-transparent fl...
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