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Assays and methods for the diagnosis of post-streptococcal disorders

a post-streptococcal disorder and diagnostic test technology, applied in the direction of biological material analysis, material analysis, instruments, etc., can solve the problem of no clear diagnostic test for these diseases

Inactive Publication Date: 2013-07-18
QUEST DIAGNOSTICS INVESTMENTS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method for diagnosing a disease in a person caused by a streptococcal infection. The method involves testing a biological sample from the person for the presence of autoantibodies that recognize specific proteins from the infection. The presence of these autoantibodies indicates a current or past infection. The method can be used to diagnose diseases such as PANDAS, post-GABHS glomerulonephritis, rheumatic fever, autism spectrum disorders, and Syndenham's chorea. The method involves testing for the presence of anti-streptococcal antibodies and reactivity of the autoantibodies to neuronal cells. The invention provides a useful tool for diagnosing and monitoring diseases caused by streptococcal infections.

Problems solved by technology

Because the molecular mechanisms underlying the disease are largely unknown, there is no clear diagnostic test for these diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Hu, Ri, and Yo Antibody Immunofluoresence Assay

[0060]Biological samples can first be obtained and tested for anti-streptococcal antibodies such as anti-streptolysin O. Eighty anti-streptolysin O (ASO) positive serum samples, 30 ASO negative serum samples, and ten normal serum samples were obtained. For the determination of ASO positive or negative, an antibody quality of 200 IU / ml was used. Samples were submitted for ASO testing without clinical information and used after de-identification.

[0061]1:40 dilutions of the patient sera was prepared in a pH buffered solution. Slides with monkey cerebellar tissues affixed thereto were removed from cold storage and kept at room temperature for five minutes. Approximately 50 μl of diluted sera and controls were applied to the reaction wells on the slides. The controls consisted of samples with antibodies determined to have reactivity to one or all of the Hu, Yo, and Ri antigens. The slides were incubated for 30 minutes at room temperature in ...

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Abstract

Provided are methods for diagnosing a disease in a subject with a previous streptococcal infection by determining the presence or absence of one or more autoantibodies in a biological sample from the subject, wherein the one or more autoantibodies recognize an antigen from a protein selected from the group consisting of ELAVL2, ELAVL3, ELAVL4, Nova-1, Nova-2, Cdr1, Cdr2; and Cdr3. The presence of such autoantibodies is indicative of a positive diagnosis for a post-streptococcal disease such as PANDAS, post-GABHS glomerulonephritis, rheumatic fever, autism and Syndenham's chorea.

Description

FIELD OF THE INVENTION[0001]The invention relates to medically useful assays and methods for the diagnosis of post-streptococcal infection complications including PANDAS, post-GABHS glomerulonephritis, rheumatic fever, and Syndenham's choreaBACKGROUND OF THE INVENTION[0002]Pediatric Autoimmune Neuropsychiatric Disorder Associated with group A Streptococcal infection (PANDAS) is characterized by pediatric onset; neuropsychiatric disorders (obsessive compulsive disorder [OCD]) and / or tic disorder; abrupt onset and / or episodic course of symptoms; association with group A hemolytic streptococcal (GABHS) infection; and association with neurological abnormalities (motor hyperactivity or adventitious movements, including choreiform movements or tics). PANDAS is one of a spectrum of post-streptococcal infection complications that include post-GABHS glomerulonephritis, rheumatic fever, and Syndenham's chorea.[0003]According to the National Institute of Mental Health (NIMH), children with PAN...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/68
CPCG01N33/6854G01N2469/20G01N33/56944G01N33/564G01N2333/315G01N2800/38
Inventor NAIDES, STANLEY J.POPOV, JOANNA
Owner QUEST DIAGNOSTICS INVESTMENTS INC
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