Composition for improving inflammatory disease using abh antigens

a technology of inflammatory diseases and antigens, applied in the direction of dna/rna fragmentation, plant/algae/fungi/lichens ingredients, dispersed delivery, etc., can solve the problems of no success in these efforts so far, and achieve the effects of improving or relieving symptoms of inflammatory diseases carrying inflammation, enhancing skin barrier function, and accelerating antimicrobial peptide expression

Inactive Publication Date: 2014-02-06
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention describes a composition that can improve symptoms of inflammatory diseases in various tissues, including skin. It helps keep skin healthy by boosting antimicrobial peptide expression and enhancing skin barrier function. It can also help heal damaged skin and prevent or reduce aging by reducing inflammation and strengthening skin elasticity, reducing wrinkles. The composition can be used for screening useful substances that can improve inflammatory skin diseases and aging.

Problems solved by technology

However, none of these efforts have been successful so far, and the function of ABH antigen itself still needs to be studied.

Method used

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  • Composition for improving inflammatory disease using abh antigens
  • Composition for improving inflammatory disease using abh antigens
  • Composition for improving inflammatory disease using abh antigens

Examples

Experimental program
Comparison scheme
Effect test

experimental example 1

Investigation of the Expression of ABH Antigen in Different Human Tissues

[0044]Biopsy was performed to obtain different human tissues from various parts of human body. Immunohistochemical staining was performed by using ABH antigen specific antibody in order to investigate the expression of ABH antigen in each tissue. Particularly, the tissues obtained from biopsy were fixed in 10% formalin for overnight. Then, formalin was removed and the tissues were dehydrated, so as to make paraffin block. The tissue block was sliced into 0.4 μm thick sections, which were placed on slide. The slide was loaded in a 58° C. dry oven for one hour to melt paraffin. To eliminate paraffin completely, the slide was treated with xylem 4 times, 5 mines for each time, and then the slide was soaked in 100% ethanol twice, 1minute for each, in 95% ethanol for 1 minute, in 80% ethanol for 1 minute, and in 70% ethanol for 1 minute, leading to stepwise rehydration. The slide was washed with running tap water for...

experimental example 2

Down-regulation of the Expression of ABH Antigen by UV Irradiation

[0046]In normal tissues, the tissues of a person with blood type A are stained only by A antigen specific antibody, which usually takes place in granular layer in epidermis (Dabelsteen et al., J Invest dermatol 82:13-17(1984)). Minimal erythema dose (MED), indicating the strength with which the first erythema is observed after UV irradiation, was measured individually. Each skin was irradiated by 2MED, and then skin biopsy was performed 0, 28, 48, and 72 hours after the UV irradiation. Immunohistochemical staining was performed by the same manner as described in Experimental Example 1 to investigate the expression of ABH antigen.

[0047]As a result, ABH antigen was normally expressed until 24 hours after the UV irradiation, However, after 48-72 hours, the expression of ABH antigen was gradually decreased (FIG. 3).

experimental example 3

Down-regulation of the Expression of ABH Antigen in Various Skin Diseases

[0048]Based on the fact that the expression of ABH antigen is decreased by UV irradiation at the strength of inducing inflammation, the expression of ABH antigen was investigated in diverse skin diseases presumably carrying symptoms caused by irregulation of inflammation reaction. For the investigation, tissues were obtained by biopsy from patients having psoriasis (type A), atpoic dermatitis (type A), ichthyosis (type A), cellulitis (type A), discoid lupus (type A), acne (type B), etc. Immunohistochemical staining was performed with the tissues by the same manner as described in Experimental Example 1 to investigate the expression of ABH antigen.

[0049]As a result, the expression of ABH antigen in granular layer was deficient in most pathological tissues. In spite of individual difference, the expression of abnormal ABH antigen was commonly in spinous layer or basal layer (FIG. 4 and FIG. 5). This result indica...

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Abstract

The invention provides a method for treating an inflammatory skin disease in a subject comprising administering to the subject an amount of composition comprising willow tree extract as an active ingredient effective to treat the inflammatory skin disease.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for improving inflammatory disease, more specifically to a composition for improving inflammatory disease to improve inflammatory reactions and the barrier function of skin, to enhance aging resistance and skin elasticity, and to prevent or treat aging.BACKGROUND ART [0002]ABH antigen is the antigen that determines ABO blood type. ABO blood type is determined by the difference of ABO gene activity in glycosylated terminal having a specific structure. ABH antigen is mainly expressed on the surface of erythrocyte, so it is a major reason that causes rejection when different type of blood is transfused. It is also known that ABH antigen is expressed in diverse tissues including blood vessel, salivary gland, sweat gland, small intestine, large intestine, and pancreas, in addition to erythrocyte. In normal skin tissues, this antigen is found mostly in granular layer where differentiation of keratinocytes is most progress...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/76A61K8/97A61Q19/08
CPCA61K36/76A61K8/97A61Q19/08A61K8/06A61K8/60A61K9/0014A61K9/0019A61K9/0095A61K9/08A61K9/107A61K9/2059A61K36/07A61K36/254A61K36/258A61K36/28A61K36/539A61K39/0005A61K47/26C12N15/1137C12N2310/14C12Y204/01041A61K8/9789A61P1/04A61P13/00A61P17/00A61P17/06A61P17/10A61P27/02A61P29/00A61P37/00A61K39/395A61K31/7105A61K9/06
Inventor OH, JANG HEEJUNG, JI-YONGLEE, DONG HUNLEE, SERAHKIM, YEON KYUNGSHIN, JEONG-EUNLEE, JUNE HYUNKYUNGCHUNG, JIN HO
Owner SEOUL NAT UNIV R&DB FOUND
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