Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods and compositions for targeted nucleic acid sequencing

a nucleic acid and composition technology, applied in the field of methods and compositions for targeted nucleic acid sequencing, can solve the problems of increasing costs, time and labor, and high cost of genomic analysis on a whole genome level, and achieve the effect of high-accuracy sequencing reaction

Inactive Publication Date: 2016-09-29
10X GENOMICS
View PDF4 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such genetic analysis on a whole genome level is costly not only monetarily but also in time and labor.
These costs increase with protocols involving analyses of separate individual DNA samples.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and compositions for targeted nucleic acid sequencing
  • Methods and compositions for targeted nucleic acid sequencing
  • Methods and compositions for targeted nucleic acid sequencing

Examples

Experimental program
Comparison scheme
Effect test

example 1

Whole Exome Capture and Sequencing: NA12878

[0138]Genomic DNA from the NA12878 human cell line was subjected to size based separation of fragments using a Blue Pippin DNA sizing system to recover fragments that were greater than or equal to approximately 10 kb in length. The size selected sample nucleic acids were then copartitioned with barcode beads in aqueous droplets within a fluorinated oil continuous phase using a microfluidic partitioning system (See, e.g., U.S. patent application Ser. No. 14 / 682,952, filed Apr. 9, 2015, and incorporated herein by reference in its entirety for all purposes), where the aqueous droplets also included the dNTPs, thermostable DNA polymerase and other reagents for carrying out amplification within the droplets, as well as DTT for releasing the barcode oligonucleotides from the beads. This was repeated both for 1 ng of total input DNA and 2 ng of total input DNA. The barcode beads were obtained as a subset of a stock library that represented barcode...

example 2

Whole Exome Capture and Sequencing: NA19701 and NA19661

[0145]Genomic DNA from the NA19701 and NA19661 cell lines was prepared according to the methods described above in Example 1. Data, including phasing data, from those two cells lines is provided in the table below:

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumesaaaaaaaaaa
volumesaaaaaaaaaa
volumesaaaaaaaaaa
Login to View More

Abstract

The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 14 / 927,297, filed Oct. 29, 2015, which claims the benefit of U.S. Provisional Application No. 62 / 072,164, filed Oct. 29, 2014, each of which is expressly incorporated herein by reference in their entirety for all purposes.BACKGROUND OF THE INVENTION[0002]The ability to sequence genomes accurately and rapidly is revolutionizing biology and medicine. The study of complex genomes, and in particular, the search for the genetic basis of disease in humans, involves genetic analysis on a massive scale. Such genetic analysis on a whole genome level is costly not only monetarily but also in time and labor. These costs increase with protocols involving analyses of separate individual DNA samples. Sequencing (and re-sequencing) of polymorphic areas in the genome that are linked to disease development will contribute greatly to the understanding of diseases, such as cancer, and therapeut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q1/6837C12Q1/6874C12Q2535/122C12Q2537/159C12Q2565/514C12Q2563/179
Inventor JAROSZ, MIRNASCHNALL-LEVIN, MICHAELSAXONOV, SERGEHINDSON, BENJAMINZHENG, XINYING
Owner 10X GENOMICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com