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Therapeutic cell washing, concentration, and separation utilizing acoustophoresis

a technology of acoustophoresis and cell washing, applied in the direction of enzymology, specific use bioreactor/fermenter, biomass after-treatment, etc., to achieve the effect of reducing cost, improving efficiency and improving process efficiency

Pending Publication Date: 2021-11-04
FLODESIGN SONICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present patent proposes a way to simplify and reduce the cost of processing particles like therapeutic cells. The new methods and systems replace or enhance conventional procedures like centrifugation and physical filtration, as well as multiple washing steps. This approach is sterile and can be performed in a continuous form. The technical effects include improved efficiency, reduced time and cost, and improved quality of processed cells.

Problems solved by technology

The first media may contain preservatives such as dimethyl sulfoxide (DMSO) which are undesirable for future applications / uses of the particles.

Method used

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  • Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
  • Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
  • Therapeutic cell washing, concentration, and separation utilizing acoustophoresis

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[0114]The ability of an acoustophoretic system of the present disclosure to concentrate Jurkat T-cells was tested. Jurkat T-cells have a diameter of 11 micrometers (μm) to 14 μm. An acoustophoretic device was used, and a Beckman Coulter Vi-CELL X was used at various test conditions to measure the cell density reduction.

[0115]In the first trial A, the T-cells were concentrated, and the cell density of the permeate was measured. The dotted line indicates the feed cell density. Desirably, the cell density in the permeate is as low as possible, indicating that the cells are retained in the concentrate. The graph in FIG. 29 shows the results of trial A over time. The results show very low cell densities in the permeate, between 0.0 and 0.2 million cells / mL, showing that most of the cells are in the concentrate. The results also show a high permeate reduction percentage, between 80% and 99%.

[0116]In the second trial B, the T-cells were concentrated, and the cell density of the permeate wa...

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Abstract

Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. patent application Ser. No. 15 / 942,427, filed on Mar. 30, 2018, which is a continuation-in-part of U.S. patent application Ser. No. 15 / 586,116, filed on May 3, 2017, now U.S. Pat. No. 10,640,760, which claims priority to U.S. Provisional Patent Application Ser. No. 62 / 330,947, filed on May 3, 2016, and to U.S. Provisional Patent Application Ser. No. 62 / 359,182, filed on Jul. 6, 2016, and to U.S. Provisional Patent Application Ser. No. 62 / 374,910, filed on Aug. 15, 2016. U.S. patent application Ser. No. 15 / 942,427, filed on Mar. 30, 2018 also claims the benefit of U.S. Provisional Patent Application Ser. No. 62 / 479,309, filed on Mar. 30, 2017. The disclosures of these applications are hereby fully incorporated herein by reference in their entirety.BACKGROUND[0002]Concentrating therapeutic cells and transferring them from one solution into another (usually referred to as washing) are two processes ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N13/00C12N1/02C12M1/00
CPCC12N13/00C12M47/04C12M47/02C12N1/02
Inventor LIPKENS, BARTDIONNE, JASONPRESZ, JR., WALTER M.DUTRA, BRIAN
Owner FLODESIGN SONICS
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