Methods of chromosome drying and spreading
a technology of chromosomes and drying methods, applied in biochemistry apparatus and processes, organic chemistry, sugar derivatives, etc., can solve the problems of often problematic incubators and continued problems in achieving achieve optimal chromosomal drying and spreading, optimal chromosomal spreading, optimal chromosomal spreading
Active Publication Date: 2011-03-01
GENECARE MEDICAL GENETICS CENT
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Problems solved by technology
Ultimately, problems continued to exist with respect to achieving optimal chromosomal drying and spreading for chromosomes of interest.
In the past, incubators were used to dry biological samples in order to isolate and spread chromosomes for analysis, however the incubators were often problematic because certain environmental conditions could not be regulated such as relative humidity, temperature, and drying times. U.S. Pat. Nos. 5,976,871 and 5,851,790 disclose a cytogenetic chamber that allows a user to regulate the chamber's environmental conditions by setting a precise conditions based on temperature, relative humidity, air flow and time.
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Embodiment Construction
[0014]In the following description, like reference characters designate like or corresponding parts throughout the several views. Also in the following description, it is to be understood that such terms as “forward,”“rearward,”“front,”“back,”“right,”“left,”“upwardly,”“downwardly,” and the like are words of convenience and are not to be construed as limiting terms.
[0015]Referring now to the drawings in general, the illustrations are for the purpose of describing a preferred embodiment of the invention and are not intended to limit the invention thereto. The present invention relates to an improved method of drying and spreading chromosomes for cytogenetic studies. In the preferred embodiment, a biological sample is prepared for treatment in a cytogenetic chamber. The method of the present invention requires first obtaining a biological sample. The type of abnormality or defect being screened will determine the necessary biological sample. For example, in order to detect prenatal dis...
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Abstract
The present invention provides for a method of drying and spreading chromosomes from various biological samples to yield optimal chromosomal spreading. The method requires preparing a biological sample for treatment, providing a cytogenetic chamber capable of setting predetermined conditions, pre-testing a portion of the biological sample in the cytogenetic chamber, and finally treating the remaining biological sample. The method is useful to yield metaphase chromosomes that are small and rounded, with very few overlapping or scattered chromosomes. Furthermore, the method is uses restricted ranges of temperature and relative humidity to achieve consistent chromosomal spreading. The morphologies of the chromosomes are preserved in order to execute banding techniques at 550 bands and chromosomal analysis on high-resolution chromosomes.
Description
BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates generally to methods of chromosome drying and spreading. Further, the present invention relates to a method of drying chromosomes from various biological samples to yield optimal chromosomal spreading.[0003]2. Description of the Prior Art[0004]With the advent of cytogenetics, the ability to analyze chromosomal material has become increasingly important. Cytogenetic analyses are widely used for peripheral blood studies, hematological malignancy investigations, screening prenatal defects, and solid tissue and solid tumor research. Peripheral blood is often analyzed to detect numerical and structural abnormalities of chromosomes. For less obvious chromosomal abnormalities, such as translocations, deletions, and duplications, chromosomal analysis having higher resolutions and more detailed banding are required. Cytogenetics may also be used for early diagnosis of diseases. In order to successfu...
Claims
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IPC IPC(8): C12Q1/68C07H21/02C07H21/04
Inventor BUCHANAN, PHILIP D.
Owner GENECARE MEDICAL GENETICS CENT