The invention discloses a brush for preparing a rat oral submucous fibrosis experimental model. The brush for preparing a rat oral submucous fibrosis experimental model comprises a brush handle, a guide rail, a brush head, multiple first bristles, a transfusion tube, a clapboard, an elastic member, a driving rod and an accelerating rod, wherein a liquid storage chamber is formed in the brush handle, an areca nut extraction solution is stored in the liquid storage chamber, one end of the transfusion tube is communicated with the liquid storage chamber, the other end of the transfusion tube is communicated with the first bristles, the elastic member elastically connecting the brush head with the clapboard, one end of the driving rod props against the brush head, the other end of the drivingrod extends into the liquid storage chamber, and the accelerating rod is formed by bending and extending from the other end of the driving rod and stretches into the transfusion tube. The brush for preparing a rat oral submucous fibrosis experimental model, provided by the invention, is convenient to operate, and good in simulation effect, and greatly saves the manpower and time cost of experimenters.