Method of extraction of saponin by non-solvent microwave heat
A solvent-free, saponin-free technology, applied in the field of saponin extraction, can solve problems such as energy consumption reduction
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Embodiment 1
[0032] Take 42g of commercially available dried American ginseng, without crushing, put it in a 250ml conical flask and irradiate it in a 950W microwave oven for 1 minute. The surface temperature of ginseng is 78°C. Add 100ml of 95% ethanol with a liquid temperature of 17°C into the conical flask for extraction for 10 minutes. Finally, pour out the ethanol, irradiate the second microwave for 45 seconds, the surface temperature of ginseng is 69°C, then extract with 100ml 95% ethanol, finish after 15 minutes, combine the two extracts, dissolve and shrink to 8ml, and dry to obtain a white powder 2.68g, the measured total saponin content is 95.48% (dry basis), and the product yield is 6.4%.
Embodiment 2
[0034] Take 400g of machine-extracted oil tea cake, crush it to a diameter of 2-5mm fine particles, put it in a 1000ml flask, and irradiate it with microwaves in a 950W microwave oven for 2 minutes. The actual temperature of the material is 91°C, quickly add 500ml of 90% ethanol, and stir for 15 minutes , when the liquid temperature reaches 34°C, filter out the ethanol, and put the filter residue into a microwave oven for 2 minutes and 10 seconds to irradiate. Ethanol was filtered off, and the two filtrates were combined. After microfiltration, it was dissolved and shrunk to about 150ml, and dried to obtain 52.8g of light yellow dry powder, with a total saponin content of 96.4% (dry basis), and a yield of 13.2%. The filter residue was irradiated in the microwave for 2 minutes and 40 seconds, the temperature of the material rose to 72°C, and 400ml of 90% ethanol was added and stirred for 15 minutes, the liquid temperature was 31°C, the liquid was filtered through microfiltratio...
Embodiment 3
[0036] Take 30g of dry tea leaves, put them into a 250ml triangular flask, irradiate them with microwaves in a 950W microwave oven for 40 seconds, the actual temperature of the material is 70°C, add 110ml of 86% ethanol, and extract for 12 minutes. Pour out and filter, and then heat the filter residue by microwave irradiation for 40 seconds. The actual temperature of the material is 74°C, add 100ml of 86% ethanol to extract for 15 minutes, separate the liquid residue, combine the two filtrates after filtration, concentrate to about 10ml, and dry to obtain 3.46g Yellow-green powder. After testing, the catechin is 89.3% (dry basis), and the product yield is 11.53%.
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