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Aerogenic enteric bacilli secreting alga-dissolving substance and its uses in myxophyceae algal tufa

An enterobacter aerogenes, algae-dissolving technology, applied in biological water/sewage treatment, bacteria, water/sludge/sewage treatment, etc., can solve the problems of high difficulty, high cost, high pollution poison and so on

Inactive Publication Date: 2008-07-16
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of high cost, high difficulty, high pollution and toxic residues in the control of water blooms in physical, chemical and engineering methods, the present invention provides a microorganism that secretes algae-dissolving substances and an application technology for using the microorganism to control cyanobacteria blooms

Method used

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Embodiment Construction

[0023] Fermentation culture test: first inoculate the Enterobacter aerogenes into the above-mentioned liquid basal medium with sodium citrate at 1% inoculation amount, culture at 37°C, measure OD650 every 2 hours, draw a growth curve, and determine the fermentation time. The fermentation equipment is a 50L BIO6000A microbial fermentation system, produced by Gaoji Biological Engineering Co., Ltd. of Shanghai University of Science and Technology. The sterilization parameters are 121°C, 20-30 minutes, and the fermentation parameters are pH5.8.0-7.0, DO 70-90, and fermentation temperature 34.0-39.0°C, stirring speed 70-130 rpm, fermentation time 16-48 hours, the fermentation medium is the above-mentioned basal medium with sodium citrate added. The fermentation broth was spray-dried under the conditions of an inlet temperature of 200°C and an outlet temperature of 73°C.

[0024] Applications. In September 2004, a test of dry powder control of Microcystis bloom was carried out at ...

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Abstract

The invention relates to an Aerobacter enterobacteria which can excrete algaelysing substances and its application in controlling blue-algae water bloom. The said strain is a bacteria which can excrete algaelysing substances and is obtained by thickening the natural water sample 10-100 times to perform algaelysing experiment separation, which is thought as Aerobacter enterobacteria W5 by biochemical determination. The white powder preparation can be obtained after the fermentation by the spray drying of Aerobacter enterobacteria, and the algaelysing substances all can effectively kill the water bloom blue algae in an application concentration of 2-200ppm. Furthermore, the said preparation is of convenient use, is safety and nontoxic, and is no harm to the environment. The condition of fermentation of the said Aerobacter enterobacteria is: the sterilizing parameter is 121 DEG C and 20-30 minutes; the fermentation parameter is: the pH is 5.8.0-7.0 and DO is 70-90, the fermentation temperature is 34.0-39.0 DEG C, the mixing speed id 70-130 r / minute, and the fermentation time is 16-48 minutes. The fermentation culture medium formulation is: in the fermentation process there is no need to supplement the materials and to adjust the PH, and the spray drying condition of the fermentation products is that the import temperature is 198-210 DEG C and the export temperature is 68-79 DEG C.

Description

technical field [0001] The invention relates to a strain of enterobacter aerogenes secreting algae-dissolving substances and its application in the control of cyanobacteria blooms. The preservation date of this strain is May 19, 2006, and the preservation number is: CCTCC NO: M206049. The classification name is: Enterobacter aerogenes W5 (Enterobacter aerogenes W5), the name of the depository unit is China Center for Type Culture Collection, and the address is Wuhan University, Wuhan, China, zip code 430072. Background technique [0002] Currently, physical, chemical and engineering methods are generally used to control water blooms. Engineering and physical methods such as flocculation algae removal, ozone algae removal, mechanical algae removal, ultraviolet light algae removal, etc.; chemical methods such as copper sulfate, chlorine dioxide, hypochlorite Salt and other algicides, etc. Engineering and physical methods often have high cost, complicated equipment, and high ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/00
Inventor 赵以军吴刚程凯廖明军
Owner HUAZHONG NORMAL UNIV
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