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Senile dementia vaccinum and preparing method thereof

An aging and vaccine technology, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, tetrapeptide components, etc., can solve problems such as poor immunogenicity and safety doubts

Inactive Publication Date: 2008-08-13
姚志彬 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] with Aβ 42 As an immunogen, the complete fragment of Aβ has the following problems: (1) Aβ 42 The peptide contains Aβ toxic fragments (25-35 amino acids), and its safety as an immunogen has been questioned; (2) The β-sheet structure of Aβ is an important form of Aβ polypeptide in Aβ deposits and senile plaques in the brain, while Synthetic Aβ 42 It needs to go through allosteric to have good immunogenicity; (3) Aβ 42 Peptide molecular weight <10000, poor immunogenicity

Method used

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  • Senile dementia vaccinum and preparing method thereof
  • Senile dementia vaccinum and preparing method thereof
  • Senile dementia vaccinum and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of synthetic peptide Aβ based on neutralizing epitope 1 42 A subunit vaccine comprising the following steps:

[0025] (1) Use a peptide solid-state synthesizer to artificially synthesize a peptide that neutralizes epitope 1

[0026] Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln(Aβ 1～15 ), column purification to more than 95%;

[0027] (2) Using the cross-linking agent MBS (M-maleimidobenzoyl-N-hydroxy succinimideester) to couple the polypeptide to the branched chain of the carrier heptalysine (heptalysine), and incubate; purify to more than 95% by HPLC chromatography; Dilute the antigen with sterile 0.01mol / L PBS (pH7.4);

[0028] (3) Mix the conjugate with an equal volume of MF59 adjuvant, and mix with a vortex shaker for about 30 minutes to prepare a vaccine.

Embodiment 2

[0029] Example 2 Preparation of synthetic peptide Aβ based on neutralizing epitope 2 42 A subunit vaccine comprising the following steps:

[0030] (1) Artificially synthesized peptides that neutralize epitope 2

[0031] Val-Gly-Gly-Val-Val-lle-Ala (Aβ 36～42 )

[0032] (2) Using the cross-linking agent MBS (M-maleimidobenzoyl-N-hydroxy succinimideester) to couple the polypeptide to the branched chain of the carrier heptalysine and incubate;

[0033] (3) The conjugate is prepared with an aluminum adjuvant to prepare a vaccine.

Embodiment 3

[0034] Example 3 Preparation of synthetic peptide Aβ based on neutralizing epitope 3 42 A subunit vaccine comprising the following steps:

[0035] (1) Artificially synthesized peptides that neutralize epitope 3

[0036] Phe-Arg-His-Asp-Ser-Gly-Tyr

[0037] (2) Using the cross-linking agent MBS (M-maleimidobenzoyl-N-hydroxy succinimideester) to couple the polypeptide to the branched chain of the carrier heptalysine and incubate;

[0038] (3) Prepare the conjugate with MF59 adjuvant to prepare a vaccine.

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Abstract

A senile dementia vaccine is prepared through artificially synthesizing at least one polypeptide containing the neutral epitope with A beta 42, coupling it to carrier protein or carrier polypeptide to become coupled substance, incubating for ageing, and applying the acceptable adjuvant to said coupled substance to obtain the A beta 42 subunit vaccine of senile dementia.

Description

technical field [0001] The invention relates to a vaccine prepared by biotechnology and a preparation method thereof, in particular to an senile dementia vaccine and a preparation method thereof. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease characterized clinically by progressive memory and loss of acquired knowledge until the patient loses the ability to live. With the increase of the number of cases, AD brings a heavy burden to the family and society, and is an important social and medical health problem. Regarding the treatment of AD, there is no ideal method at present. The existing treatment strategies mainly focus on relieving symptoms, but do not prevent the pathological process of AD, and their clinical efficacy is not satisfactory. Aβ is the main component of senile plaques in the pathological changes of senile dementia, which consists of 39-42 amino acids. Recent studies have shown that the accumulation and deposition of A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K45/00A61K38/10A61K38/07A61K38/08A61K39/39A61K47/42A61P25/28A61K39/385
Inventor 姚志彬
Owner 姚志彬