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Corn borer resistant transgenic ultra-sweet corn regeneration system and construction method thereof

A super-sweet corn, a technology for establishing a method, which can be applied to other methods of inserting foreign genetic materials, plant regeneration, horticultural methods, etc., and can solve the problem of weak embryo viability, difficulty in tissue culture and transgenic research, and ultra-sweet corn embryo lactose content. Advanced problems, to achieve efficient and stable results

Inactive Publication Date: 2009-06-10
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Super-sweet corn has high endosperm sugar content and weak embryo viability, making its tissue culture and transgenic research difficult. Ke Xiayi et al. have used the electric shock method to transform the B.t.

Method used

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  • Corn borer resistant transgenic ultra-sweet corn regeneration system and construction method thereof
  • Corn borer resistant transgenic ultra-sweet corn regeneration system and construction method thereof
  • Corn borer resistant transgenic ultra-sweet corn regeneration system and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The method of the invention is used for the development of super-sweet corn transgenic plants, and can obtain highly efficient and stable regenerated plants. The invention includes the induction of super-sweet corn callus, the establishment of embryogenic callus and the development technology of corn borer-resistant transgenic super-sweet corn using it as a receptor.

[0050] The detailed establishment process of corn borer-resistant transgenic super sweet corn regeneration system is as follows:

[0051] (1) Induction of super-sweet corn callus: Take the super-sweet corn inbred line 1132 ear that has been pollinated and grown for 10 days. Young ears are surface-sterilized with 70% alcohol, rinsed with sterile water for 1-2 times, then soaked in 15-20% (v / v) sodium hypochlorite solution for 25 minutes, and rinsed with sterile water for 3-4 times. Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, inoculate them in induction medium,...

Embodiment 2

[0060] The detailed establishment process of corn borer-resistant transgenic super sweet corn regeneration system is as follows:

[0061](1) Induction of super-sweet corn callus: Take super-sweet corn single-cross Yuetian No. 3 ears that have been pollinated and grown for 9 days. At this time, the length of immature seeds is 1.0-2.0mm. Bract leaves and young ears are surface-sterilized with 75% alcohol, rinsed with sterile water for 1-2 times, then soaked in 10-15g / L calcium hypochlorite solution for 20 minutes, and rinsed with sterile water for 3-4 times. Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, inoculate them in induction medium, and culture induced calluses in the dark at 25-27°C.

[0062] (2) Establishment of embryogenic callus: the above-mentioned immature embryos were grown on the induction medium for 3 to 4 weeks, and light yellow, dense, granular, and dry embryogenic calli were picked as transformation recipients or tran...

Embodiment 3

[0069] The detailed establishment process of corn borer-resistant transgenic super sweet corn regeneration system is as follows:

[0070] (1) Induction of super-sweet corn callus: Take super-sweet corn inbred line 1132 ears that have been pollinated and grown for 11 days. At this time, the immature embryos of immature seeds are 1.0-2.0 mm in length, and the outer bracts of the ears are carefully removed. Young ears are surface-sterilized with 72% alcohol, rinsed with sterile water for 1-2 times, soaked in 15-20% (v / v) sodium hypochlorite solution for 21 minutes, and rinsed with sterile water for 3-4 times. Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, inoculate them in induction medium, and culture induced calluses in the dark at 25-27°C.

[0071] (2) Establishment of embryogenic callus: the above-mentioned immature embryos were grown on the induction medium for 3 to 4 weeks, and the embryogenic callus with light yellow color, dense,...

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Abstract

The present invention provides one kind of maize borer resisting transgenic super sweet maize regeneration system and its establishment method. The establishment method includes the following steps: inducing callus of super sweet maize, establishing embryonic callus, genetic gun transformation of embryonic callus cell, culture and screening of the transformed callus, and plant differentiation and transplanting. The method has high super sweet maize cell transforming regeneration rate, and the present invention provides one kind of super sweet maize cell transforming regeneration system and can obtain transgenic regenerated plant at high efficiency and high stability.

Description

technical field [0001] The invention relates to biotransgenic technology, in particular to a corn borer-resistant transgenic super-sweet corn regeneration system and its establishment method. Background technique [0002] Super sweet corn belongs to sweet corn (Zea mays L. Sacchara sturt), which enjoys the name of vegetable and fruit corn, and has become an important cultivated crop in the world today. The finished product is one of the important consumer foods in the United States, Japan, Western Europe and some countries and regions in Southeast Asia. South China is the main producing area of ​​super sweet corn in my country, and super sweet corn has become a characteristic and dominant crop in Guangdong. However, the corn borer is extremely harmful to super sweet corn, resulting in a decline in yield and quality. The application of pesticides will cause environmental pollution, ecological balance damage, and increase planting costs. At present, the super sweet corn vari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N5/04C12N15/87A01H4/00
Inventor 李余良胡建广
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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