Tissue culture breeding method for clonic fibraurea stem

A clone and tissue culture technology, which is applied in the field of cultivation and transplantation of yellow vine, can solve the problems of slow propagation speed, low seed germination rate, and large seasonal influence, achieving no seasonal restrictions, huge economic benefits, and broad application prospects Effect

Inactive Publication Date: 2009-07-15
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention, i.e. the purpose of the present invention, is to overcome the shortcomings of the prior art seed propagation method that are greatly affected by the season, low seed germination rate, and slow propagation speed, and provide a method for tissue culture propagation of Huangteng clone

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] The tissue culture room of the Institute of Tropical Forestry collected the explants of the tiller buds from the Longdong Institute of Guangzhou and the Jinjikeng Forest Farm in Gaoyao for many times. Using the method of the present invention, the tiller buds were sawed off from the mother plant with a saw, and the tiller buds were explanted The size of the body is 15-40cm high. By adopting the two disinfection steps of the present invention, the highest disinfection success rate can reach 59.1%, and the average success rate is 34.9%-38.4%. The specific method and steps are as follows;

[0022] 1) Acquisition of tillering bud explants, the key to collecting tillering bud explants is to prevent external injury of buds and shock cracking of bud cores. If the sprouts have wounds, the soil can easily enter the sprouts and cannot be sterilized successfully. After the bud heart is shattered, the wound will brown rapidly, which will easily lead to the death of the bud heart. ...

Embodiment 2

[0034] The successfully sterilized explants adopt the anti-browning induction medium and proliferation medium of the present invention, and cluster buds can be seen in 2-3 months, and the cluster bud induction rate is about 10%. After subculture for 45-50 days / generation , The multiplication factor is 3-4 times.

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Abstract

The present invention relates to the tissue culture propagation method of the clonal line of Huangteng, which is characterized in that: 1) the collection of the tillering bud explants, preventing the trauma of the buds and the shock of the bud core during the process of collecting the tillering bud explants, and Make the height of the tillering bud explant be 15-40cm; 2) the disinfection of explant, adopt twice disinfection method, at first, clean explant with washing powder, the second step: disinfect with 0.1% mercuric chloride 1-2 minutes, rinse 3 times with sterile water; 3) prevent browning, use cysteine ​​or ascorbic acid solution to wash the aforementioned tillering bud explants; 4) cluster bud induction and subculture; 5) rooting culture , The sprouts with a height greater than 2cm are most suitable for rooting culture. This method uses the tiller buds as explants, which can reproduce the excellent clones through selection and measurement, and produce seedlings of good varieties, which have strong market competitiveness, so this technology has broad application prospects and can produce huge economic benefits.

Description

Technical field [0001] The invention relates to a method for cultivating yellow vine, in particular to a method for tissue culture and propagation of yellow vine clones. The invention belongs to the technical field of cultivation and transplantation of tropical trees, especially yellow rattan. Background technique [0002] Yellow vine (Daemonorops margaritae Beccari.) is a thorny, climbing, clustered vine plant of the genus Daemonorops of the Calamoideae family (Calamoideae) in the palm family (Palmae), and is a species in tropical and subtropical regions of South China. Companion plants endemic to forests. The cane has good crafting properties and is an excellent raw material for making furniture and crafts. Teng tender shoots are rich in various nutrients needed by the human body and can be eaten as vegetables. The fruit can be extracted "Kirin's Blood Dragon". The seeds are hard and are the traditional material for making "Buddha beads". Therefore, Huangteng is a mul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02P60/40
Inventor 尹光天曾炳山裘珍飞李湘阳刘英许煌灿
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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