Method for asexually breeding seedlings of Sargassum polycystum

A technology of sargassum and seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of difficult long-term mass production of seedlings, seedling production restrictions, etc., and achieves easy control of cultivation conditions and is not affected by seasons Restricted, short-period effects

Inactive Publication Date: 2016-07-13
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of Sargassum sargassum seedlings mainly relies on sexual reproduction, but due to the influence of seasons, the production of seedlings is greatly restricted, and it is difficult to produce large-scale seedlings for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of PESI culture medium

[0024] Take 20ml of PESI stock solution and dilute to 1000ml with autoclaved seawater. PESI storage solution composition: NaNO 3 350mg, Na 2 glycerophosphate·5H 2 O50mg, Na 2 -EDTA25mg, FeCl 3 ·6H 2 O0.25mg, H 3 BO 3 5 mg, MnCl 2 1mg, ZnCl20.125mg, CoCl 2 0.025mg, Tris500mg, KI0.1mg mixed, distilled to 100ml with distilled water, pH value is 7.6, then autoclaved and stored in the refrigerator.

Embodiment 2

[0025] Example 2: Cleaning of Sargassum stolonifer samples

[0026] Select fresh algal bodies of well-grown Sargassum sargassum, use a brush to remove the miscellaneous algae and attachments on the surface of the algae under a dissecting microscope, and then rinse repeatedly with sterilized filtered seawater for 4-6 times.

Embodiment 3

[0027] Embodiment 3: temporary cultivation of Sargassum sargassum samples

[0028] Before tissue culture, place the algae at 20°C, light intensity 2000lx, and photoperiod 14D:10L; the culture medium is sterilized filtered seawater, containing 0.10mol / L NaNO 3 and 0.05mol / LNaH 2 PO 4 .

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Abstract

The invention discloses a method for asexually breeding seedlings of Sargassum polycystum and belongs to the technical field of algal tissue culture. The method comprises: cleaning and temporarily cultivating collected wild Sargassum polycystum, sterilizing complete tender side shoots with sodium hypochlorite, using stems or leaves of the side shoots as explants to induce adventitious buds, culturing the adventitious buds to form regenerated leaves, using the regenerated leaves as explants to form massive adventitious buds by successive multi-generation induction, and subjecting the adventitious buds to in-vitro culture to induce rhizoid, forming complete regenerated seedlings for artificial cultivation. A method for inducing the adventitious buds comprises: placing cuttings of the stems or leaves in PESI medium, and culturing for 7-10 days at 20-25 DEG C under illumination intensity of 1000-4000 lx with a ratio of light to dark hours being 8-10:16-14; a method for inducing the rhizoid comprises: placing the adventitious buds in PESI medium with 0.1-0.5 mg / L of 6-BA, and culturing for 20-25 days at 24-27 DEG C under illumination intensity of 1500-3000 lx with a ratio of light to dark hours being 10-12:14-12.The regenerated leaves obtained herein can serve as explants in successive multiple generations to induce adventitious buds, grow in a high quantity and are easy to store and not bound by seasons in seedling production.

Description

technical field [0001] The invention relates to a method for raising seedlings by tissue culture of Sargassum sargassum, belonging to the technical field of seaweed tissue culture. technical background [0002] Sargassum spp. is a genus of Phaephyta (Phaephyta), most of which are warm-water species, widely distributed in warm and warm waters. Seaweed of the genus Sargassum has high economic value. After processing, alginic acid, iodine crystals, mannitol and high-activity dietary fiber can be extracted. It is an important industrial raw material. Sargassum seaweed is rich in nutrients such as protein, fat, amino acid, multivitamins, calcium, potassium, magnesium, manganese, zinc, iodine, phosphorus and selenium, and some unique active substances, such as seaweed polysaccharide, alginic acid, high Unsaturated fatty acids, etc., also have great development potential in food, medicine, health care and breeding production. In addition, Sargassum grows rapidly and has a large s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 朱军鲍时翔苏醒邹潇潇林勇
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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