Calamus simplicifolius clone tissue culture and fast propagation method

Abstract

The invention relates to a group-cultivation quick-propagation method for single-leaf calami, which is characterized in that asexual organs such as sprouting buds and the like of single-leaf calami are utilized for group cultivation and quick propagation. The specific procedure is as follows, first, sprouting buds are sawed from a stock plant with a saw in sunny days to prevent bud bodies from being damaged and the bud cores from being shattered; second, the explants of the sprouting buds are sterilized; third, anti-browning process is done to the explants of the sprouting buds to prevent browning; fourth, clumpy buds are formed by induction on a clumpy buds induction medium, after the buds are formed, the clumpy buds are transferred to a breeding culture medium for breeding and propagation; fifth, bud seedlings meeting the height requirement are cultivated on a radication culture medium for radication, bud seedlings not meeting the height requirement are first cultivated for reinforcement to grow to the required height and then are cultivated for radication; sixth, seedlings are transplanted to fields, after transplant, bactericidal agent is sprayed to the seedlings and shading nets are utilized to shade the seedlings until the seedlings are planted out of gardens. The invention solves the technical problem of group cultivation and quick propagation of the single-leaf calami clone and is applied in the breeding of the single-leaf calami clone and the quick propagation of elite seedlings, which not only promotes the development of artificial plant production of rattans andhelps to achieve good economic benefit.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of a single-leaf dwarf vine clone, which is suitable for tissue culture and rapid propagation of plants of the genus A. genus Palmaceae. It belongs to the technical field of tissue culture of clone plants. Background technique [0002] Calamus simplicifolius Wei. is a thorny, climbing, clumping vine of the genus Calamus in the subfamily Calamoideae of the palm family (Palmae), and is a tropical, southern Asian species. A companion plant endemic to tropical forests. The cane has good crafting properties and is an excellent raw material for making furniture and crafts. Teng tender shoots are rich in various nutrients needed by the human body and can be eaten as vegetables. The seeds are hard and are the traditional material for making "Buddha beads". Therefore, vine is a multi-purpose precious forest plant with high economic value and development prospect, and it is also the m...

AI Technical Summary

Problems solved by technology

However, it is very difficult to study the tissue culture technology of palm plants. After the research of the two five-year plans, only the embryos can be used as explants to achieve rapid tissue culture and rapid propagation, while the terminal buds, sprouting buds, roots, leaves, etc. If asexual organs are used as explants, tissue culture and rapid propagation cannot be successful

Therefore, the original rattan tissue culture technology is only limited to seedling propagation, that is, industrialized rapid propagation of seedlings through tissue culture, but it is still unable to propagate clonal seedlings

Since the original tissue culture technology uses seed embryos as explants, the genetic basis of the propagation material cannot be completely controlled, and the seedlings propagated have no quality difference compared with the seedlings, but the cost of tissue culture seedlings is higher than that of seed propagation seedlings , it is difficult to compete with the seedlings bred by seeds in the market, so the original tissue culture technology with embryonic explants has little application prospect

Images