High efficiency quick breeding method for cichorium intybus test tube seedling

A chicory and efficient technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of complicated procedures, long cultivation period, easy to mutate, etc., and achieve the effect of increasing the reproduction coefficient and reducing the cultivation cost.

Inactive Publication Date: 2009-07-15
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation cycle is long, the cost is high, the procedure is complicated, the plant regeneration frequency is low, and the mutation is easy to occur

Method used

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  • High efficiency quick breeding method for cichorium intybus test tube seedling
  • High efficiency quick breeding method for cichorium intybus test tube seedling
  • High efficiency quick breeding method for cichorium intybus test tube seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: choose the plump chicory seeds, rinse with tap water, soak and shake for 30s with 70% ethanol, 0.1% HgCl 2 Disinfect the surface for 12 minutes, rinse with sterile water 6 times, inoculate on 1 / 2 MS basic medium, light for 16 hours a day, light intensity 2000lx, culture temperature 25±2℃.

[0048] Cut the base of chicory aseptic seedlings cultured for 20 days and inoculate them with 2.0mg / L6-BA, 0.5mg / LNAA, 10mg / L AgNO 3 , 100mg / L V B1 , 300mg / L proline and 4% sucrose MS medium, 25±2°C, 2500lux light culture for 7 days, the surface of the explant and the incision will gradually produce green adventitious buds, after 15 days, the buds develop Clustered adventitious buds, the number of adventitious buds formed by each leaf is at most 44, with an average of 36.08 (such as figure 1 shown). Cut off the adventitious buds more than 2cm, transfer to the MS medium that adds 0.5mg / L IBA, produce adventitious roots after 7 days, can form 3 robust adventitious roots ...

Embodiment 2

[0050] Embodiment 2: choose the plump chicory seeds of grain, wash with tap water, soak and shake 30s with 70% ethanol, 0.1%HgCl 2 Disinfect the surface for 12 minutes, rinse with sterile water 6 times, inoculate on 1 / 2 MS basic medium, light for 16 hours a day, light intensity 2000lx, culture temperature 25±2℃,

[0051] Cut the middle and upper part of the leaves of chicory aseptic seedlings cultured for 30 days, and inoculate them in the supplementary 1.5mg / L6-BA, 0.5mg / L NAA, 5mg / L AgNO 3 , 50mg / L V B1 , 300mg / L proline and 4% sucrose MS medium, 25±2°C, 2500lux light culture for 7 days, the surface of the explant and the incision will produce green adventitious buds one after another, about 15 days, the buds develop Clustered adventitious buds, the number of adventitious buds formed by each leaf is at most 35, with an average of 29.06 (such as figure 2 shown). Cut out adventitious buds over 2cm, transfer them to 1 / 2MS medium supplemented with 0.5mg / L IBA, 5-7d will prod...

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PUM

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Abstract

The invention discloses a rapid propagation method of chicory tissue culture seedlings. Its technical feature is to use chicory leaves as explants, including the following steps: (1) generation of sterile seedlings; (2) direct generation of adventitious buds from leaves in vitro; (3) rooting, training of seedlings and transplantation of adventitious buds plant. In the present invention, the leaves of chicory aseptic seedlings are cut into pieces as explants, cultured for 15-20 days, 20-40 adventitious buds can be directly formed on each explant, and more than 30 robust tissue-cultured seedlings can be regenerated within 35-40 days , a sterile seedling with 5 leaves can produce about 500 high-quality tissue culture seedlings after in vitro culture for 30 days. Compared with the conventional isolated plant regeneration system, the culture cycle is shortened by at least 25-30 days, the culture cost is reduced by more than half, and the reproduction coefficient is increased by 3-5 times.

Description

technical field [0001] The invention relates to a method for rapid propagation of chicory tissue-cultured seedlings, in particular to a method for rapid propagation of chicory tissue-cultured seedlings through the direct generation of adventitious buds from isolated leaves, and belongs to the field of biological group cultured seedlings. Background technique [0002] Chicory (Cichorium intybus.L) is a perennial herb of the genus Chicory in the Compositae family. It is not only a high-yield and high-quality forage, but also a new high-end characteristic vegetable. In addition, chicory contains inulin, horse chestnut bark, horse chestnut bark, and wild lettuce As a coffee substitute, it has the functions of preventing and treating jaundice hepatitis, cardiovascular disease, osteoporosis, etc. It has the development of feeding, food and medicine. potential. There have been some reports on chicory tissue culture in recent years [(1) Varotts, lucchin M, Parrinip.Immature embryos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 韩晓玲贾敬芬
Owner NORTHWEST UNIV
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