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Polyploid breeding method for inducing heterogenous tripoid scallop by physical means

A technology of polyploid breeding and physical methods, applied in the field of hybrid scallop polyploid breeding, can solve the problems of cytochalasin B carcinogenicity, limited wide application, high price, etc.

Inactive Publication Date: 2009-08-26
OCEAN UNIV OF CHINA
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Problems solved by technology

Although the induction rate of chemical methods is high, close to 100% in some species, cytochalasin B is carcinogenic, expensive, insoluble in water, and 6-DMAP also has potential threats to human health, which limits their Wide application in production

Method used

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Embodiment Construction

[0008] This method is to dry the parent clam scallops for 40 minutes and place them in filtered seawater at 20°C to stimulate ovulation; at the same time, dry the parent scallops for one hour in the shade, place them in filtered seawater at 16°C to stimulate ovulation, and In filtered seawater at 20°C, the eggs (Chlamys farreri) and the sperm produced by the parent scallop (Patinopectenyesoensis) were mixed and fertilized at a ratio of 1:5. After fertilization, when 30% of the eggs have been discharged from the second polar body, a pressure of 25 MPa is applied for 18 minutes, and the treated fertilized eggs are incubated according to a conventional method to obtain triploid scallops.

[0009] The scallops incubated by the method can obtain about 59% allotriploids in the scallops in the scallop cycle stage. D-type larvae have a triploid rate of 10-40%. Although the triploid rate is lower than that of chemical induction, there is no harm to health during operation and consumpt...

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Abstract

The invention relates to a physical method of polyploidy-breeding by introducing allotriploid scallops, put female scallops in filter seawater at the temperature of 16íµ after being dried in shade for 40 minutes to stimulate ovulation; put male scallops in filter seawater at the temperature of 16íµ after being dried in shade for 1 hour to stimulate sperm-drain, and then put said ovums and sperms produced in filter seawater at the temperature of 20íµ for fertilization following the mixing rate of 1í†5. When 30% of ovums have been drained out of second polar bodies after fertilization, force pressure of 20-30 MP for 20-10 minutes, hatch fertilized ovums according to the conventional method and get triploid scallops. The scallops cultured in said invention are characterized on fast,growth, high infertility and fresh texture, could make up for the shortages of low production and induced texture during the multiplication periods caused by diseases and high-density culturing in recent years.

Description

technical field [0001] The invention relates to a breeding method for hybrid scallop polyploids, in particular to using physical methods to induce allotriploid scallops to carry out polyploid breeding. Background technique [0002] In recent years, diseases and high-density breeding have resulted in reduced production and reduced meat quality during breeding. [0003] Shellfish triploids are widely used for their growth advantages, high sterility, and excellent meat quality. Large-scale production has been achieved in various shellfish such as Pacific oyster, American oyster, etc. Small-scale experiments have confirmed that the triploid growth rates of clams such as Chlamys farreri and bay scallop are higher than that of corresponding diploids in different proportions. [0004] There are usually chemical methods for artificially inducing triploid shellfish, such as cytochalasin B, 6-DMAP, etc. Physical methods such as temperature shock, hydrostatic pressure. Although the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N5/06A01K67/033C12N15/01
Inventor 包振民汪小龙胡景杰陈刚王珏
Owner OCEAN UNIV OF CHINA
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