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Method for quick in vitro reproducing excellent quality cushaw

A pumpkin, in vitro technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of browning of explants, high pollution rate, low cell biomass, etc., and achieve the speed of accelerated reproduction. Effect

Inactive Publication Date: 2007-07-18
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN118233C discloses a method for rapid propagation of pumpkin tissue culture industrialized seedlings, but this method has the limitation of variety genotype, and secondly limited by the variety genotype, the differentiation rate of buds is relatively low, and the pollution rate is relatively high, and Browning of explants is easy to occur. In the later process of cell culture using callus, the cell biomass is not too high. These have limitations for cell culture and production of secondary metabolites using pumpkin tissue.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Materials: Pumpkin seeds Jin Li, Red Li Wang, Sheng Li.

[0021] Select pumpkin seeds that are plump and of the same size, Jinli, Hongliwang, and Shengli, respectively, and perform the following operations: 1.1 Disinfection method for explant materials: Soak the seeds in constant temperature water at 25°C for 7h to 8h. Then, on the ultra-clean bench, disinfect the surface with 70% alcohol for 30 seconds, and place 0.1% HgCl 2 The solution was sterilized for 4-8 minutes respectively, and the effect of different sterilizing time on the germination of sterile vaccines was observed. Shake constantly during disinfection, and then rinse with sterile water 4 to 5 times. After the seeds are sterilized, the seeds are respectively inserted into the seed germination medium under aseptic conditions and cultivated. The basic medium is MS with 0.8% agar and 2% sucrose, and the pH value is adjusted to 5.8. The temperature of the incubator is 25±1°C, and the culture is carried out i...

Embodiment 2

[0034] Choose the pumpkin seed Jinli as material, the operation steps are as in Example 1, and each medium is also the same as in Example 1, except that the explant cotyledons, hypocotyls, See Table 1 for the induction of callus by radicle.

[0035] Table 1 Induction of callus by explants in different parts

[0036] Sample site

Embodiment 3

[0038] Get pumpkin seed Jin Li as material, operation steps such as embodiment 1, get cotyledon when callus induction and do explant induction callus, the induction medium composition of callus in the step (2) is as shown in table 2, Other culture medium is composed with embodiment 1, and the results are shown in Table 2.

[0039] Table 2 Induction of callus by different hormone concentrations

[0040] Medium composition

[0041] It can be seen from the table that when the combination of NAA and 6-BA induced pumpkin callus, the induction rate was the highest with MS+6-BA2.0+NAA 0.2.

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Abstract

A fast in vitro reproduction method of the high-quality pumpkin includes such steps as preparing the aseptic seedling of pumpkin, inducing calli in the culture medium (MS + 6BA + NAA), bud differentiating culture in the culture medium (MS + BA+ NAA), and rooting culture in the culture medium (1 / 2MS + NAA).

Description

(1) Technical field [0001] The invention relates to a method for in vitro rapid propagation of pumpkins, through which large-scale production of excellent seedlings can be carried out. (2) Background technology [0002] Pumpkin, scientific name Cucurbita spp, is an annual vine herb of Cucurbitaceae Cucurbita. According to its origin and character, it can be divided into Chinese pumpkin (commonly known as nest melon, pumpkin, etc.), Indian pumpkin (commonly known as wax gourd, stir melon, etc.) and American pumpkin (commonly known as zucchini). In recent years, it has been found that pumpkin has great value in nutrition, health care and medical treatment. Pumpkin is rich in starch, fat, reducing sugar, various amino acids, vitamins and minerals. Modern studies have shown that pumpkins also contain various functional factors such as cancer prevention and disease treatment, so they have received more and more attention. The cultivation and conventional breeding of pumpkins ha...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 邹克琴张拥军楼纪东
Owner CHINA JILIANG UNIV
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