Method for preparing tetrahydroxy diphenyl ethylene glycoside separated from fleece-flower root

Abstract

This invention relates to a method for separating and preparing tetrahydroxy distyrene glucoside from Polygonum multiflorum. The method comprises: (1) pulverizing Polygonum multiflorum tubers, sieving to 20 mesh, extracting with water or ethanl, and concentrating the extract; (2) separating the concentrated extract with polyamide or macroporous adsorbent resin chromatographic column, eluting with ethanol, detecting by thin-layer chromatography, and collecting distyrene glucoside component; (3) vacuum-concentrating, preparing saturated solution at room temperature, cooling slowly, evaporating the solvent to precipitate crystals, vacuum-filtering, and recrystallizing to obtain white needle-like crystals of distyrene glucoside. The method has such advantages as easy operation, and high product purity.

Description

technical field

[0001] The patent of the present invention relates to the field of phytochemistry, the separation and preparation process of natural active ingredients in Chinese herbal medicine. Background technique

[0002] At present, the reports about stilbene glycosides in Radix Polygoni Multiflori are all white amorphous substances. As for the separation of pure stilbene glycosides, the literature reports all select different kinds of chromatographic supports and repeat column chromatography for many times to finally obtain pure stilbene glycosides.

[0003] For example, in the separation of stilbene glycosides, ethanol reflux extraction is used in China, the ethanol extract is recovered, and then extracted by ether cold soaking, and the pH gradient method is used to extract emodin, emodin methyl ether and other components from the ether extract. The alcoholic extract extracted with ether is refluxed with water and ethyl acetate for several times, and then repeatedly ...