Quick breeding method for clove strain í«Luolanzií»

Abstract

The invention relates to a method for quickly cultivating the cloves, which comprises that: inducing the germ section of cloves in the cultivate medium which contains 0.5-1.5mg/L BAP and 0.05-0.15mg/L ZT for 3-4 weeks, then arranging the induced fresh sheath into the cultivate medium which contains 0.5-1.5mg/L IAA+1-3mg/L azoles to root, to obtain the seed of cloves. The invention can quickly differentiate the explant via inducing the hormone, to improve 4-5 times of number in one month.

Description

technical field [0001] The invention relates to a rapid propagation method of a clove variety 'Luo Lanzi'. Background technique [0002] The lilac variety 'Luo Lanzi' is a lilac variety cultivated by artificial hybridization in the Botanical Garden of the Institute of Botany, Chinese Academy of Sciences, using the North China lilac native to China as the female parent and the European lilac variety 'Buddha's lilac' as the male parent. In the breeding, due to the use of the native native lilac germplasm with strong stress resistance, North China lilac, and the double-petaled European lilac variety with strong ornamental value as the hybrid parents, the resulting variety 'Luo Lanzi' not only has strong flower color and flower shape. It has excellent ornamental characters such as fullness, compact inflorescence, and dense flowering. At the same time, compared with the exotic clove varieties introduced in recent years, it has more outstanding resistance to humidity, heat, cold, ...

AI Technical Summary

Problems solved by technology

Objectively speaking, due to the particularity of endogenous hormones and secondary meristem differentiation of Syringa plants, the proliferation efficiency of fine varieties of this genus has been restricted for a long time.

At the end of the 20th century, there were occasional reports on the bud explant culture of European clove (Refouvelet et al.1998). In addition, because the tissue culture of this kind of woody plant is no matter whether it is multiplied in the bottle, or it is transplanted out of the bottle or grown in the open field. Due to the limitations of factors such as slow accumulation of seedlings and long seedling childhood, the work of rapid propagation by tissue culture is not extensive enough.

However, in the above-mentioned process of using tissue culture to rapidly propagate clove seedlings, there are both common problems faced by tissue culture and rapid propagation of woody plants, as well as specific problems of this genus.

The main manifestations are as follows: 1. The combination of exogenous hormones and the concentration ratio of the clove varieties obtained from the same hybrid combination are very different in the proliferation medium for rapid proliferation of shoots after inoculation of bud explants; There are many seedlings; 3. The seedlings in the bottle are too high, and it is easy to lodging when planting out of the bottle

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