Culture medium and method for inducing embryogenic callus of Southeast-Asia wild rice selfing line to differentiate and grow seedling

Abstract

The invention provides a culture medium and a method for inducing an embryogenic callus of a Southeast-Asia wild rice selfing line to differentiate and grow a seedling. By using the culture medium provided by the invention, a quick propagation system is established aiming at a Southeast-Asia ordinary wild rice selfing line; multiple seedlings can be quickly differentiated and grown within 60 days by adopting the culture medium provided by the invention to cultivate the callus of the Southeast-Asia ordinary wild rice selfing line.

Description

technical field [0001] The invention relates to a culture medium and a method, in particular to a culture medium and a method for inducing the embryogenic callus of the Southeast Asian wild rice inbred line to differentiate into seedlings. Background technique [0002] Plant dedifferentiation and differentiation are affected by many factors, such as genotype, explant selection, medium type and the interaction between them, but so far, it has not been possible to establish a "universal culture" suitable for all genotypes. base” (Bolibok, 2006). However, the genome of wild rice is quite different from that of cultivated rice, so the culture system suitable for cultivated rice cannot produce the same positive effect on all wild rice. For example, the in vitro culture of medicinal wild rice is limited by genotype, and it is difficult to obtain embryogenic callus of medicinal wild rice (Ding Yumei, 2003); the efficiency of high stem wild rice leaves to induce callus is low (Ni W...

AI Technical Summary

Problems solved by technology

[0002] Plant dedifferentiation and differentiation are affected by many factors, such as genotype, explant selection, medium type and the interaction between them, but so far, it has not been possible to establish a "universal culture" suitable for all genotypes. Base" (Bolibok, 2006)

However, the genome of wild rice is quite different from that of cultivated rice, so the culture system suitable for cultivated rice cannot have the same positive effect on all wild rice

For example, the in vitro culture of medicinal wild rice is limited by genotype, and it is difficult to obtain embryogenic callus of medicinal wild rice (Ding Yumei, 2003); the efficiency of high-stem wild rice leaves to induce callus is low (Ni Wenjin, 2007); however, mature plants can be differentiated from cryopreserved callus of Oryza sativa (Zeliang, 2010)

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