39results about How to "Rapid differentiation" patented technology

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention relates to a biologic polypeptide medicine blood vessel support and a preparation method thereof. The biologic polypeptide medicine blood vessel support comprises a support body and active medicines. The support body which is medical material with pores and good biocompatibility is made from stainless steel, cobalt-base alloy, titanium alloy, nickel-titanium alloy or polylactic acid biopolymer; the active medicines comprise a biologic polypeptide medicine and a smooth muscle cell proliferation inhibition medicine. The support is characterized in that: the support body with the pores has the biologic polypeptide medicine fixed on the internal surface of the body and the smooth muscle cell proliferation inhibition medicine coated on the external surface of the body. The preparation me(3) carrying out the after-treatment of the surface of the support body; (4) preparing the medicines; (5) coating the external surface; (6) fixing the medicine onto the internal surface; (7) carrying out the process step of low temperature drying. The support can selectively absorb endothelium progenitor cells which quickly differentiate into endothelium cells to promote the restoration of the endothelium after the support is built in; the support can also effectively inhibit the proliferation and migration of vascular smooth muscle cells, persistently and effectively reduce the formation of a new inner membrane, effectively prevent restenosis inside the support, and avoid the risk of potentially fatal late thrombosis.

The invention discloses a composite biological active ceramic material of Apatite-Wollastonite / beta-tricalcium phosphate (AW / beta-TCP), which comprises the following steps: adopting sol-gel process to prepare AW former powder; preparing TCP former powder through chemical sediment method; sieving TCP former powder through 60-order standard sieve; putting AW former powder and TCP former particle in the material mixing machine according to different proportion; adding certain rate corresponding to powder weight geoceric acid or naphthalene particle with grain size at 20-30 order; making 5wt% polyvinyl alcohol solution as adhesive; blending completely; moulding; drying; sintering to obtain porous product.

The invention discloses a 3D model constructed in vitro by means of a serum-free culture medium as well as a construction method of the 3D model and relates to the technical field of biological materials in tissue engineering. Due to selection variety of seed cells and good in-vitro construction repeatability, industrial preparation of the 3D model can be realized. The model is an in-vitro model formed by primary corneal epithelial cells subjected to tissue isolation, limbal stem cells, immortalized corneal epithelial cells, primary oral epithelial cells or oral mucosa squamous cancer cell lines TR146 subjected to in-vitro amplification and stratified through the serum-free culture medium with an undersurface-gas-liquid surface staged culture method.

The invention relates to the technical field of sweet osmanthus grafting and seedling breeding, in particular to a sweet osmanthus grafting and seedling breeding method. Sweet osmanthus seedlings subjected to layering serve as stock, leaf bud seedlings obtained after leaf buds are subjected to cuttage and seedling breeding serve as scions, processing and control are conducted in the breeding process of the scions and the stock, so that the content of harmless bacteria on the stock and the scions is small, influences of the harmful bacteria on the cut environment in the grafting process are avoided, and affinity of the stock and scions is improved. The sweet osmanthus seedlings subjected to layering serve as the stock, and the leaf bud seedlings obtained after the leaf buds are bred serve as the scions so that after the stock and the scions are grafted, regeneration can be conducted quickly, the speed of healing of the stock and the scions is increased, and the survival rate is increased.

The invention relates to a method for quickly cultivating the cloves, which comprises that: inducing the germ section of cloves in the cultivate medium which contains 0.5-1.5mg / L BAP and 0.05-0.15mg / L ZT for 3-4 weeks, then arranging the induced fresh sheath into the cultivate medium which contains 0.5-1.5mg / L IAA+1-3mg / L azoles to root, to obtain the seed of cloves. The invention can quickly differentiate the explant via inducing the hormone, to improve 4-5 times of number in one month.

The invention discloses a relieving and repairing composition containing various plant extracts, and belongs to the technical field of cosmetics formulas. The relieving and repairing composition is prepared from the following components in percentages by weight: 1-10% of a moisturizer, 3-10% of a relieving and repairing agent, 0.1-3% of a preservative and the balance of deionized water. The relieving and repairing agent comprises hydrolyzed lupine protein, a long carob fruit extract and a jasminum officinale extract. The composition has a remarkable relieving and repairing effect, can effectively relieve problems of redness, swelling, paining and itching, papule and desquamation and the like of skin after the skin is irritated, meanwhile can rapidly repair a damaged barrier function of theskin, and improves the ability of the skin resisting harm of the outside. According to difference of dosage forms of products, the suggested adding amount of the relieving and repairing composition is 2-8%, and the composition has the advantages of naturalness, no irritation, obvious relieving and repairing effect and the like.

The invention relates to the technical field of river channel treatment, and discloses an efficient desilting device for water conservancy river channel project treatment. The device comprises a ship body, wherein a chain bucket machine is fixedly connected to the top part of the right end of the ship body; a bucket is arranged at the bottom end of the chain bucket machine, and a grating is fixedly connected to the inner wall of the bucket; a box body is arranged at the inner bottom part of the ship body; a primary filtering area, a first-stage precipitating area and a second-stage precipitating area are sequentially arranged in the box body from right to left; and a pre-precipitating groove is formed in the bottom part of the primary filtering area and communicates with the first-stage precipitating area. According to the device, the second-stage precipitating area and a water collecting groove are arranged, so that the advantage of greatly reducing water pollution is realized; river water and silt can be effectively separated after being treated in the second-stage precipitating area, and thus the silt can be quickly settled; after being separated through an inclined pipe separating assembly, the separated river water is collected into the water collecting groove, so that reasonable output water quality is ensured.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

The invention discloses a reproduction method of Boston fern by tissue culture of sprout and aims at providing a reproduction method of Boston fern by tissue culture of sprout with high reproduction speed and low cost. The reproduction method includes the steps of (a) induction and differentiation step of sprout; (b) propagation culture step of germ-free sprout; and (c) domestication transplanting step of germ-free sprout. The method puts the root hair of Boston fern in MS culture medium added with 3 percent of white sugar water without adding agar, thus being capable of effectively promoting the quick differentiation of the root hair of Boston fern to form round carmus. As the method adopts 0.25-0.8mg / L 6-benzyladenine (BA) 3 percent of sugar, and 0.25-0.8mg / L MS culture medium of kinetin (KT) with as propagation culture medium, the round carmus can propagate vigorously. Finally, the round carmus is put in the MS culture medium with 0.5-1.5mg / L 3- indolebutyric acid (IBA) and 3 percent of sugar, and then the round carmus can quickly root all around. The reproduction method can be widely applied to the field of tissue culture of sprout of Boston fern.

The invention discloses a medicament and a method for promoting branch lignification and flower bud differentiation of green prickleyash. The medicament is prepared from the following components: uniconazole, 28-epihomobrassinolide, monopotassium phosphate, a potassium-calcium-boron-magnesium-iron-zinc microelement water-soluble fertilizer and water. The method is clear in agent application, stage and position, and is easy to master by green prickleyash planting owners; the medicament is combined with manual regulation; the drug effect duration is moderate, the drug effect half-life period is short, and no phytotoxicity or residue is generated; the lignification and flower bud differentiation of the green prickleyash are rapid, the internode length is moderate, single clusters have many fruits and moderate density, and oil cells are large and full; zanthoxylum schinifolium is high in yield and good in economic benefit.

The invention relates to the technical field of fruit tree planting, in particular to a planting method of mountain citrus. The planting method includes the steps of land selection for planting, young tree management, fruiting tree management and the like. Compared with a traditional method, the planting method has the advantages that by overall specification of citrus planting, fertilizing and modes of application, nutritional needs at different periods are guaranteed, normal citrus growth is achieved, the citrus is moderate and stable in yield, high in fruit setting rate, developed in roots, fast in young fruit growth, and less in use of chemical pesticides; with the method, ripe fruit harvested is moderate in size, plump and bright, high in content of soluble solid, dense in flavor, high in sweetness, juicy and easy in slag melting and high in yield and quality, and pollution-free planting standard is met.

The invention relates to the technical field of Chinese herbal medicine planting, in particular to a high-yield propagation expanding method of wild ganoderma lucidum. Before propagation expanding, ganoderma lucidum mycelia are taken and inoculated to an original ganoderma lucidum root nutrient bar; compared with a mode of taking mycelia from fungal context tissue of a yellow white growth area ofa ganoderma lucidum pileus or fungal context tissue in the middle of a pileus on a ganoderma lucidum stipe, the survival rate of ganoderma lucidum can be greatly increased to 98% or above, and the damage to original ganoderma lucidum thalli is greatly reduced; before the ganoderma lucidum mycelia are inoculated and cultured, a planting pit is effectively utilized as a fixed fungal bed, auxiliary strains are inoculated in advance, in cooperation with auxiliary materials and leaves, long-time buried fermentation is conducted in the pit, a fungal bed environment with sufficient nutrients is prepared accordingly, in the later period, the ganoderma lucidum mycelia are inoculated to the fermented fungal bed, the fungal bed can provide sufficient and suitable nutrients for the ganoderma lucidum mycelia, the ganoderma lucidum mycelia are quickly differentiated, the growth vigor of ganoderma lucidum is greatly improved, the yield of ganoderma lucidum is increased, and the method is unmatched bythe prior art.

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention discloses a propagation method for coral grass seedling tissue culture, and aims to provide a propagation method for coral grass seedling tissue culture with high propagation speed and low cost. The propagation method comprises the following steps: (a) a step of induction and differentiation of buds; (b) a propagation culture step of sterile seedlings; and (c) an acclimation and transplantation step of the sterile seedlings. The propagation method adopts an MS culture medium of 0.5 to 1.5 mg / L of 6BA and 0.05 to 0.15 mg / L of 2,4-D to effectively promote quick differentiation of coral grass leaves to form adventitious buds, and adopts the propagation culture in an MS culture medium of 0.3 to 1 mg / L of 6BA and 3 percent of sugar to propagate the adventitious buds exuberantly; and finally, the propagation seedlings are placed in an MS culture medium of 0.8 to 1.5 mg / L 3IBA and 3 percent of sugar, and the periphery of the propagation seedlings can quickly take roots. The propagation method can be widely applied to the field of coral grass seedling tissue culture.

The invention discloses an in-vitro simulated organoid cultivation method for colon cancer cells.The in-vitro simulated organoid cultivation method sequentially includes steps of sterilizing hollow fibers; preliminarily laying collagen on the hollow fibers; injecting Caco-2 cell suspension into inner cavities of the hollow fibers, arranging the hollow fibers in incubators at the temperatures of 37+ / -0.2 DEG C, and driving the hollow fibers by driving devices to axially rotate at the rotational speeds of 90 degrees / hour for the rotational time of 4 h so as to uniformly attach cells; arranging the hollow fibers with the cultivated cells in cultivation plates, adding 2-3 mL of cultivation media into each hole in each cultivation plate, arranging the cultivation plates in the incubators at the temperatures of 37+ / -0.2 DEG C and cultivating the cells; changing the cultivation media once every 1-2 days.The in-vitro simulated organoid cultivation method has the advantages that small intestinal cell hollow fiber membrane reactors capable of simulating micro-morphology of human tissues can be constructed by the in-vitro simulated organoid cultivation method, and the in-vitro simulated organoid cultivation method can be applied to research on functional component transport and absorption behavior.

The invention discloses a propagation method for coral grass seedling tissue culture, and aims to provide a propagation method for coral grass seedling tissue culture with high propagation speed and low cost. The propagation method comprises the following steps: (a) a step of induction and differentiation of buds; (b) a propagation culture step of sterile seedlings; and (c) an acclimation and transplantation step of the sterile seedlings. The propagation method adopts an MS culture medium of 0.5 to 1.5 mg / L of 6BA and 0.05 to 0.15 mg / L of 2,4-D to effectively promote quick differentiation of coral grass leaves to form adventitious buds, and adopts the propagation culture in an MS culture medium of 0.3 to 1 mg / L of 6BA and 3 percent of sugar to propagate the adventitious buds exuberantly; and finally, the propagation seedlings are placed in an MS culture medium of 0.8 to 1.5 mg / L 3IBA and 3 percent of sugar, and the periphery of the propagation seedlings can quickly take roots. The propagation method can be widely applied to the field of coral grass seedling tissue culture.

The invention relates to the technical field of milk product processing, in particular to a preparation method of pumpkin seed and buckwheat sugar-free yoghourt. The preparation method comprises the following steps: firstly, selecting fresh pumpkin seeds and buckwheat and pretreating the pumpkin seeds and the buckwheat; then, mixing fresh milk with the pretreated raw materials, adding a xylitol solution, and performing uniform mixing; then, performing filtering, performing standing and precipitating, and performing filtering again; the sealing filtrate, rapidly raising the temperature, and keeping the temperature constant for a period of time; performing cooling, when a certain cooling temperature is reached, rapidly inoculating the mixed liquid with the mixed strains in a sterile environment, then performing uniform mixing, performing canning into a sterile container, and performing constant-temperature fermentation for a period of time in a fermentation box; and finally, cooling a fermented product, and refrigerating the cooled product in a refrigerator to promote the formation of the unique flavor of the yoghourt, so as to prepare the pumpkin seed and buckwheat sugar-free yoghourt. The preparation process is simple, and the prepared pumpkin seed and buckwheat sugar-free yoghourt is rich in nutrition and meets the nutritional requirements of people with hypertension, hyperglycemia and hyperlipidemia.

The invention discloses a covered stent, which comprises a support structure and a tectorial membrane unit, and is characterized in that: the tectorial membrane unit comprises an inner-layer tectorial membrane and an outer-layer tectorial membrane which coats the support structure; the outer-layer membrane coats on the outer side of the inner-layer tectorial membrane; the inner-layer tectorial membrane is a biological membrane with good blood compatibility, and the outer-layer tectorial membrane is a biological membrane which is easy to induce the formation of thrombus; the inner-layer tectorial membrane contacting blood has good blood compatibility, can effectively reduce the formation of the thrombus, and maintains normal blood flowing channels; and the outer-layer tectorial membrane can induce the formation of the thrombus in aneurysm or thrombus of bleeding parts, promotes the aneurysm to be quickly activated and reduced, reduces the mass effect in vivo, simultaneously effectivelyadheres endothelial cells, promotes differentiation and proliferation of cells, quickens the endothelialization of lesion parts of vessels, and can more effectively treat vascular diseases such as the aneurysm and the like.

The invention belongs to the field of plant biotechnologies. By the design of a culture medium, rapid propagation and differentiation of a cymbidium rhizome are realized. The method comprises the following steps: inoculating a sterile cymbidium rhizome into a proliferation culture medium; proliferating by 9 times; transferring into a differentiation culture medium; and implementing differentiation of a seedling after 15 days to obtain a cymbidium test-tube plantlet. In the method, two culture mediums are used, all used ingredients are conventional culture medium ingredients, and browning rate of the rhizome and the culture mediums can be greatly reduced. By the method, culture period is short, and costs for the culture mediums, labor, water and electricity can be saved. The method is suitable for industrialized seedling production of cymbidium, and the problems of low propagation efficiency, long period and the like in the prior art are solved.

The invention relates to the technical field of sweet osmanthus grafting and seedling breeding, in particular to a sweet osmanthus grafting and seedling breeding method. Sweet osmanthus seedlings subjected to layering serve as stock, leaf bud seedlings obtained after leaf buds are subjected to cuttage and seedling breeding serve as scions, processing and control are conducted in the breeding process of the scions and the stock, so that the content of harmless bacteria on the stock and the scions is small, influences of the harmful bacteria on the cut environment in the grafting process are avoided, and affinity of the stock and scions is improved. The sweet osmanthus seedlings subjected to layering serve as the stock, and the leaf bud seedlings obtained after the leaf buds are bred serve as the scions so that after the stock and the scions are grafted, regeneration can be conducted quickly, the speed of healing of the stock and the scions is increased, and the survival rate is increased.

The invention discloses a soothing and repairing composition containing various plant extracts, which belongs to the technical field of cosmetic formulations. The soothing and repairing composition is made of the following components by weight: moisturizing agent 1%-10%, soothing and repairing agent 3%-10%, preservative 0.1%-3%, and the balance is ionized water. The soothing and repairing agent includes hydrolyzed lupine protein, carob fruit extract and Sufanghua flower extract. The composition has a remarkable soothing and repairing effect, can effectively relieve redness, swelling, pain, itching, papules and desquamation after the skin is stimulated, and can quickly repair the damaged barrier function of the skin and improve the ability of the skin to respond to external damage. According to different dosage forms of the product, the recommended addition amount of the soothing and repairing composition is 2%‑8%. The composition has the advantages of natural non-irritation, obvious soothing and repairing effect, and the like.

The invention provides a plant tissue culture method, which uses an AtNAC58 gene transformant to promote rapid differentiation of plant tissues, can break through the limitation of explant material selection in a traditional scheme, can use a mature plant material as an explant, and can shorten the plant tissue differentiation time, Therefore, the difficulty of plant tissue culture is reduced, and the success rate and efficiency of plant tissue culture are improved.

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

The invention provides a culture medium and a method for inducing an embryogenic callus of a Southeast-Asia wild rice selfing line to differentiate and grow a seedling. By using the culture medium provided by the invention, a quick propagation system is established aiming at a Southeast-Asia ordinary wild rice selfing line; multiple seedlings can be quickly differentiated and grown within 60 days by adopting the culture medium provided by the invention to cultivate the callus of the Southeast-Asia ordinary wild rice selfing line.

The invention relates to the technical field of river course management, and discloses a high-efficiency dredging device for water conservancy river project treatment, including a hull, the top of the right end of the hull is fixedly connected with a chain bucket machine, and the bottom end of the chain bucket machine is equipped with A bucket, the inner wall of the bucket is fixedly connected with a grid, the inner bottom of the hull is provided with a box, and the inside of the box is sequentially provided with a primary filter area, a primary sedimentation area and a secondary filter area from right to left. In the sedimentation area, a pre-sedimentation tank is provided at the bottom of the primary filtration area, and is communicated with the primary sedimentation area through the pre-settling tank. The high-efficiency dredging device for water conservancy and river project control has achieved the advantages of greatly reducing water pollution through the secondary sedimentation area and the sump. Through the treatment in the secondary sedimentation area, it can effectively decompose the river water and silt, so that the silt can be quickly recovered. Settling, and after the separation, the river water is separated by the inclined tube separation component and then poured into the sump, which can ensure reasonable effluent water quality.

The invention relates to a chondrogenic differentiation culture medium and application thereof. The chondrogenic differentiation culture medium is composed of a basic culture medium and additive components, and comprises the components of an inorganic salt component, an amino acid component, a vitamin component, a microelement component, carbohydrate, TGF-[beta]3, BMP-6, BMP-2, human serum albumin, dexamethasone, magnesium ascorbyl phosphate, recombinant human insulin, recombinant human transferrin, selenic acid and linoleic acid. The invention further provides application of the chondrogenicserum-free differentiation culture medium for mesenchymal stem cells. The chondrogenic differentiation culture medium is definite in component, free of animal-derived components and serum and safer, and the components can efficiently induce differentiation of the mesenchymal stem cells to chondrocytes under the synergistic effect.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The invention belongs to the technical field of washing products, and particularly relates to a bacteriostatic dishwashing liquid capable of strongly emulsifying edible oil and a preparation method thereof.The bacteriostatic dishwashing liquid capable of strongly emulsifying the edible oil comprises a component A, a component B, a component C, a component D, a component E and a component F, the component A comprises the following raw materials in percentage by mass: 5-10% of a super emulsifier, 6-12% of fatty alcohol-polyoxyethylene ether ammonium sulfate and 1-5% of dodecyl dimethyl amine oxide, and the component D comprises the following raw materials in percentage by mass: 0.1-0.3% of triclosan and 0.2-0.5% of alcohol. By adding the super emulsifier, the fatty alcohol-polyoxyethylene ether ammonium sulfate and the dodecyl dimethyl amine oxide, the dishwashing liquid can achieve the purpose of rapid decontamination and cleaning, and meanwhile, by adding the triclosan and the alcohol, the dishwashing liquid has relatively good antibacterial performance, and meanwhile, the solubility of the dishwashing liquid to an oil agent is also effectively improved.