39results about How to "Rapid differentiation" patented technology

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention relates to a biologic polypeptide medicine blood vessel support and a preparation method thereof. The biologic polypeptide medicine blood vessel support comprises a support body and active medicines. The support body which is medical material with pores and good biocompatibility is made from stainless steel, cobalt-base alloy, titanium alloy, nickel-titanium alloy or polylactic acid biopolymer; the active medicines comprise a biologic polypeptide medicine and a smooth muscle cell proliferation inhibition medicine. The support is characterized in that: the support body with the pores has the biologic polypeptide medicine fixed on the internal surface of the body and the smooth muscle cell proliferation inhibition medicine coated on the external surface of the body. The preparation me(3) carrying out the after-treatment of the surface of the support body; (4) preparing the medicines; (5) coating the external surface; (6) fixing the medicine onto the internal surface; (7) carrying out the process step of low temperature drying. The support can selectively absorb endothelium progenitor cells which quickly differentiate into endothelium cells to promote the restoration of the endothelium after the support is built in; the support can also effectively inhibit the proliferation and migration of vascular smooth muscle cells, persistently and effectively reduce the formation of a new inner membrane, effectively prevent restenosis inside the support, and avoid the risk of potentially fatal late thrombosis.

The invention discloses a composite biological active ceramic material of Apatite-Wollastonite/beta-tricalcium phosphate (AW/beta-TCP), which comprises the following steps: adopting sol-gel process to prepare AW former powder; preparing TCP former powder through chemical sediment method; sieving TCP former powder through 60-order standard sieve; putting AW former powder and TCP former particle in the material mixing machine according to different proportion; adding certain rate corresponding to powder weight geoceric acid or naphthalene particle with grain size at 20-30 order; making 5wt% polyvinyl alcohol solution as adhesive; blending completely; moulding; drying; sintering to obtain porous product.

The invention discloses a 3D model constructed in vitro by means of a serum-free culture medium as well as a construction method of the 3D model and relates to the technical field of biological materials in tissue engineering. Due to selection variety of seed cells and good in-vitro construction repeatability, industrial preparation of the 3D model can be realized. The model is an in-vitro model formed by primary corneal epithelial cells subjected to tissue isolation, limbal stem cells, immortalized corneal epithelial cells, primary oral epithelial cells or oral mucosa squamous cancer cell lines TR146 subjected to in-vitro amplification and stratified through the serum-free culture medium with an undersurface-gas-liquid surface staged culture method.

The invention relates to the technical field of sweet osmanthus grafting and seedling breeding, in particular to a sweet osmanthus grafting and seedling breeding method. Sweet osmanthus seedlings subjected to layering serve as stock, leaf bud seedlings obtained after leaf buds are subjected to cuttage and seedling breeding serve as scions, processing and control are conducted in the breeding process of the scions and the stock, so that the content of harmless bacteria on the stock and the scions is small, influences of the harmful bacteria on the cut environment in the grafting process are avoided, and affinity of the stock and scions is improved. The sweet osmanthus seedlings subjected to layering serve as the stock, and the leaf bud seedlings obtained after the leaf buds are bred serve as the scions so that after the stock and the scions are grafted, regeneration can be conducted quickly, the speed of healing of the stock and the scions is increased, and the survival rate is increased.

The invention relates to a method for quickly cultivating the cloves, which comprises that: inducing the germ section of cloves in the cultivate medium which contains 0.5-1.5mg/L BAP and 0.05-0.15mg/L ZT for 3-4 weeks, then arranging the induced fresh sheath into the cultivate medium which contains 0.5-1.5mg/L IAA+1-3mg/L azoles to root, to obtain the seed of cloves. The invention can quickly differentiate the explant via inducing the hormone, to improve 4-5 times of number in one month.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The invention discloses a reproduction method of Boston fern by tissue culture of sprout and aims at providing a reproduction method of Boston fern by tissue culture of sprout with high reproduction speed and low cost. The reproduction method includes the steps of (a) induction and differentiation step of sprout; (b) propagation culture step of germ-free sprout; and (c) domestication transplanting step of germ-free sprout. The method puts the root hair of Boston fern in MS culture medium added with 3 percent of white sugar water without adding agar, thus being capable of effectively promoting the quick differentiation of the root hair of Boston fern to form round carmus. As the method adopts 0.25-0.8mg/L 6-benzyladenine (BA) 3 percent of sugar, and 0.25-0.8mg/L MS culture medium of kinetin (KT) with as propagation culture medium, the round carmus can propagate vigorously. Finally, the round carmus is put in the MS culture medium with 0.5-1.5mg/L 3- indolebutyric acid (IBA) and 3 percent of sugar, and then the round carmus can quickly root all around. The reproduction method can be widely applied to the field of tissue culture of sprout of Boston fern.

The invention relates to the technical field of fruit tree planting, in particular to a planting method of mountain citrus. The planting method includes the steps of land selection for planting, young tree management, fruiting tree management and the like. Compared with a traditional method, the planting method has the advantages that by overall specification of citrus planting, fertilizing and modes of application, nutritional needs at different periods are guaranteed, normal citrus growth is achieved, the citrus is moderate and stable in yield, high in fruit setting rate, developed in roots, fast in young fruit growth, and less in use of chemical pesticides; with the method, ripe fruit harvested is moderate in size, plump and bright, high in content of soluble solid, dense in flavor, high in sweetness, juicy and easy in slag melting and high in yield and quality, and pollution-free planting standard is met.

The invention relates to the technical field of Chinese herbal medicine planting, in particular to a high-yield propagation expanding method of wild ganoderma lucidum. Before propagation expanding, ganoderma lucidum mycelia are taken and inoculated to an original ganoderma lucidum root nutrient bar; compared with a mode of taking mycelia from fungal context tissue of a yellow white growth area ofa ganoderma lucidum pileus or fungal context tissue in the middle of a pileus on a ganoderma lucidum stipe, the survival rate of ganoderma lucidum can be greatly increased to 98% or above, and the damage to original ganoderma lucidum thalli is greatly reduced; before the ganoderma lucidum mycelia are inoculated and cultured, a planting pit is effectively utilized as a fixed fungal bed, auxiliary strains are inoculated in advance, in cooperation with auxiliary materials and leaves, long-time buried fermentation is conducted in the pit, a fungal bed environment with sufficient nutrients is prepared accordingly, in the later period, the ganoderma lucidum mycelia are inoculated to the fermented fungal bed, the fungal bed can provide sufficient and suitable nutrients for the ganoderma lucidum mycelia, the ganoderma lucidum mycelia are quickly differentiated, the growth vigor of ganoderma lucidum is greatly improved, the yield of ganoderma lucidum is increased, and the method is unmatched bythe prior art.

The invention discloses an in-vitro simulated organoid cultivation method for colon cancer cells.The in-vitro simulated organoid cultivation method sequentially includes steps of sterilizing hollow fibers; preliminarily laying collagen on the hollow fibers; injecting Caco-2 cell suspension into inner cavities of the hollow fibers, arranging the hollow fibers in incubators at the temperatures of 37+/-0.2 DEG C, and driving the hollow fibers by driving devices to axially rotate at the rotational speeds of 90 degrees/hour for the rotational time of 4 h so as to uniformly attach cells; arranging the hollow fibers with the cultivated cells in cultivation plates, adding 2-3 mL of cultivation media into each hole in each cultivation plate, arranging the cultivation plates in the incubators at the temperatures of 37+/-0.2 DEG C and cultivating the cells; changing the cultivation media once every 1-2 days.The in-vitro simulated organoid cultivation method has the advantages that small intestinal cell hollow fiber membrane reactors capable of simulating micro-morphology of human tissues can be constructed by the in-vitro simulated organoid cultivation method, and the in-vitro simulated organoid cultivation method can be applied to research on functional component transport and absorption behavior.

The invention relates to the technical field of milk product processing, in particular to a preparation method of pumpkin seed and buckwheat sugar-free yoghourt. The preparation method comprises the following steps: firstly, selecting fresh pumpkin seeds and buckwheat and pretreating the pumpkin seeds and the buckwheat; then, mixing fresh milk with the pretreated raw materials, adding a xylitol solution, and performing uniform mixing; then, performing filtering, performing standing and precipitating, and performing filtering again; the sealing filtrate, rapidly raising the temperature, and keeping the temperature constant for a period of time; performing cooling, when a certain cooling temperature is reached, rapidly inoculating the mixed liquid with the mixed strains in a sterile environment, then performing uniform mixing, performing canning into a sterile container, and performing constant-temperature fermentation for a period of time in a fermentation box; and finally, cooling a fermented product, and refrigerating the cooled product in a refrigerator to promote the formation of the unique flavor of the yoghourt, so as to prepare the pumpkin seed and buckwheat sugar-free yoghourt. The preparation process is simple, and the prepared pumpkin seed and buckwheat sugar-free yoghourt is rich in nutrition and meets the nutritional requirements of people with hypertension, hyperglycemia and hyperlipidemia.

The invention provides a plant tissue culture method, which uses an AtNAC58 gene transformant to promote rapid differentiation of plant tissues, can break through the limitation of explant material selection in a traditional scheme, can use a mature plant material as an explant, and can shorten the plant tissue differentiation time, Therefore, the difficulty of plant tissue culture is reduced, and the success rate and efficiency of plant tissue culture are improved.

The invention provides a culture medium and a method for inducing an embryogenic callus of a Southeast-Asia wild rice selfing line to differentiate and grow a seedling. By using the culture medium provided by the invention, a quick propagation system is established aiming at a Southeast-Asia ordinary wild rice selfing line; multiple seedlings can be quickly differentiated and grown within 60 days by adopting the culture medium provided by the invention to cultivate the callus of the Southeast-Asia ordinary wild rice selfing line.

The invention belongs to the technical field of washing products, and particularly relates to a bacteriostatic dishwashing liquid capable of strongly emulsifying edible oil and a preparation method thereof.The bacteriostatic dishwashing liquid capable of strongly emulsifying the edible oil comprises a component A, a component B, a component C, a component D, a component E and a component F, the component A comprises the following raw materials in percentage by mass: 5-10% of a super emulsifier, 6-12% of fatty alcohol-polyoxyethylene ether ammonium sulfate and 1-5% of dodecyl dimethyl amine oxide, and the component D comprises the following raw materials in percentage by mass: 0.1-0.3% of triclosan and 0.2-0.5% of alcohol. By adding the super emulsifier, the fatty alcohol-polyoxyethylene ether ammonium sulfate and the dodecyl dimethyl amine oxide, the dishwashing liquid can achieve the purpose of rapid decontamination and cleaning, and meanwhile, by adding the triclosan and the alcohol, the dishwashing liquid has relatively good antibacterial performance, and meanwhile, the solubility of the dishwashing liquid to an oil agent is also effectively improved.