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Propagation method for coral grass seedling tissue culture

A technology of tissue culture and coral grass, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high cost, high production cost, medium cost, slow reproduction speed, etc., and achieve low cost and simplified culture conditions Or the effect of cultivation method and fast reproduction speed

Inactive Publication Date: 2012-07-18
珠海市园艺研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, this process needs more than four months, and needs higher production cost (comprising culture medium cost and culture condition consumption) etc., so existing coral grass seedling propagating technology still has following defect: propagation speed is slower, higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction and differentiation steps of buds: take the young leaves of Coryne spp. for one day as explants; the leaves are first treated with 75% alcohol for 8 seconds, and then soaked in 0.1% mercuric chloride solution for 8 minutes Take it out, and then wash it with sterile water for 4 to 5 times; take the leaves sterilized by mercuric chloride and cut them into small pieces of 1 cm, put them into 6 benzylaminopurine (6BA) and 0.05 mg / L added with 0.5 mg / L In the MS medium of 2,4-dichlorophenoxyacetic acid (2,4-D), when the blade is put into the culture medium, keep the leaf surface of the cut blade upward; the culture temperature is 24~26 degrees, and the light 1800lux, 10 hours of light per day, after 6 weeks of cultivation, it can be seen that adventitious buds are differentiated from the incision of the leaves.

[0013] (2) Proliferation and cultivation of sterile seedlings: get the adventitious buds differentiated and place 6 benzylaminopurine (6BA) of 0.3mg / L...

Embodiment 2

[0018] (1) Induction and differentiation steps of buds: take the young leaves of Coryne spp. that have just been launched for two days as explants; the leaves are first treated with 75% alcohol for 10 seconds, and then soaked in 0.1% mercuric chloride solution for 10 seconds. Take it out every minute, and then wash it with sterile water for 4 to 5 times; take the leaves sterilized by mercuric chloride and cut them into small pieces of 1 cm, put them in 6 benzylaminopurine (6BA) and 0.1 mg / L In the MS medium of 2,4-dichlorophenoxyacetic acid (2,4-D), when the blade is put into the culture medium, keep the leaf surface of the cut blade upward; the culture temperature is 24~26 degrees, and the light 2000lux, 12 hours of light per day, after 4 weeks of cultivation, it can be seen that adventitious buds are differentiated from the cuts of the leaves.

[0019] (2) Proliferation and cultivation of aseptic seedlings: get the adventitious buds differentiated and place them in 1 mg / L 6 ...

Embodiment 3

[0022] (1) Induction and differentiation steps of buds: take the young leaves of Coryne spp. which have just been expanded for two days as explants; the leaves are first treated with 75% alcohol for 12 seconds, and then soaked in 0.1% mercury solution for 12 seconds. Take it out every minute, and then rinse it with sterile water for 5 times; get the leaves sterilized by mercuric chloride and cut them into small pieces of 1 cm, put them into 6 benzylaminopurine (6BA) with 1.5 mg / L and 0.15 mg / L In the MS culture medium of 2,4-dichlorophenoxyacetic acid (2,4-D), when the blade is put into the culture medium, keep the leaf surface of the cut blade upward; the culture temperature is 24~26 degrees, and the light is 2000lux , the light time is 14 hours per day, and after 4 weeks of cultivation, it can be seen that adventitious buds are differentiated from the incision of the leaves.

[0023] (2) Proliferation and cultivation of aseptic seedlings: get the adventitious buds differenti...

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PUM

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Abstract

The invention discloses a propagation method for coral grass seedling tissue culture, and aims to provide a propagation method for coral grass seedling tissue culture with high propagation speed and low cost. The propagation method comprises the following steps: (a) a step of induction and differentiation of buds; (b) a propagation culture step of sterile seedlings; and (c) an acclimation and transplantation step of the sterile seedlings. The propagation method adopts an MS culture medium of 0.5 to 1.5 mg / L of 6BA and 0.05 to 0.15 mg / L of 2,4-D to effectively promote quick differentiation of coral grass leaves to form adventitious buds, and adopts the propagation culture in an MS culture medium of 0.3 to 1 mg / L of 6BA and 3 percent of sugar to propagate the adventitious buds exuberantly; and finally, the propagation seedlings are placed in an MS culture medium of 0.8 to 1.5 mg / L 3IBA and 3 percent of sugar, and the periphery of the propagation seedlings can quickly take roots. The propagation method can be widely applied to the field of coral grass seedling tissue culture.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a propagation method of coral grass seedling tissue culture. Background technique [0002] Coral grass, also known as twining lily, is the flower of Bamboo-leaf Auspicious Grass of Commelinaceae. Bamboo-leaf Auspicious Grass ("Plant Name and Reality Picture Test") is a climbing herb, 0.5 to 1 meter long, with alternate single leaves and narrowly lanceolate Shaped, 5-10 cm long, 1.2-1.5 cm wide, apex long acuminate, base broadly cuneate, entire; petiole 3-6 mm long, leaf sheath clasping. Flowers are purple, polygamous, in terminal panicles, with many male flowers, born in the upper part of the inflorescence; sepals 3, lanceolate-ovate; petals linear, as long as calyx; stamens 6, with hairs at the base; female flowers few, Born at the base of the inflorescence, larger than the male flowers, contained in the spathe-shaped leaf axils, with 3 rudimentary stamens; the ovary is cylindrical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 金剑平徐红文方德姚慧芬巫仕荣黄皑冰
Owner 珠海市园艺研究所
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