A kind of Chunlan rhizome tissue culture rapid propagation method

A technology of tissue culture and rhizome, applied in the field of plant biology, can solve the problems of high browning rate, long period of Chunlan tissue culture, high production cost, etc., to prevent browning, promote rapid differentiation into buds, and low browning rate Effect

Active Publication Date: 2018-04-24
WUHAN INST OF BIOENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies in the prior art that the Chunlan tissue culture period is long, the browning rate is high, and the production cost is high, and provides a Chunlan rhizome tissue culture rapid propagation method

Method used

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  • A kind of Chunlan rhizome tissue culture rapid propagation method
  • A kind of Chunlan rhizome tissue culture rapid propagation method
  • A kind of Chunlan rhizome tissue culture rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] [Example 1] Prepare two kinds of medium:

[0024] Proliferation medium (A): 1 / 2MS+NAA 9mg / L+banana juice 50g / L+agar 7g / L+sucrose 30g / L+activated carbon 1g / L, pH5.8; (B): 1 / 2MS+NAA 9mg / L+banana Juice 50g / L+pear juice 100g / L+kiwi fruit juice 100g / L+agar 7g / L+sucrose 30g / L+activated carbon 1g / L, pH5.8.

[0025] Differentiation medium (C): 1 / 2MS+NAA 0.5mg / L+6-BA 0.1mg / L+agar 7g / L+sucrose 30g / L, (D): 1 / 2MS+NAA 0.5mg / L+6-BA0 .1mg / L+vitamin C 0.45mg / L+vitamin E 0.05mg / L+sodium citrate 1g / L+potassium sorbate 0.15g / L+agar 7g / L+sucrose 30g / L, pH 5.8.

[0026] Proliferation medium preparation (take one liter as an example): (A) medium: Take about 300ml of distilled water and heat it. When small bubbles appear, add 7g of agar, stir evenly, after the dissolution is complete, then add 30g of sucrose and 1 / 2MS 2.37g, stir until completely dissolved, add plant growth regulator NAA 9mg; (B) medium: on the basis of (A) medium, add 50g of bananas, 100g of pears, 100g of kiwi, squeeze th...

Embodiment 2

[0028] [Example 2] Chunlan Rhizome Tissue Culture Rapid Propagation

[0029] 1. Prepare sterile rhizomes: Prepare sterile, well-grown rhizomes with branches, about 2 mm in diameter, cut into 1 cm lengths for later use.

[0030] 2. Chunlan rhizome multiplication:

[0031] Proliferation of rhizomes: transfer 1cm-long Chunlan rhizomes into proliferation medium (A) and (B) respectively, 1g per bottle, wipe off the water and medium with sterile paper towels, and weigh them evenly Inoculate on the surface of the medium, 25°C, light 2000Lx, 12 hours / day. After 60 days, take out the rhizomes in the bottle to wipe the water and residual medium and weigh them, and record the weight of the respective rhizomes on (A) and (B) medium. (B) rhizome weight on the medium increases 9.23 times than when inoculating before 60 days, and (A) rhizome weight on the medium only increases 2.48 times than when inoculating before 60 days, as figure 1 shown.

[0032] 3. Rhizome differentiation of Chunl...

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Abstract

The invention belongs to the field of plant biotechnologies. By the design of a culture medium, rapid propagation and differentiation of a cymbidium rhizome are realized. The method comprises the following steps: inoculating a sterile cymbidium rhizome into a proliferation culture medium; proliferating by 9 times; transferring into a differentiation culture medium; and implementing differentiation of a seedling after 15 days to obtain a cymbidium test-tube plantlet. In the method, two culture mediums are used, all used ingredients are conventional culture medium ingredients, and browning rate of the rhizome and the culture mediums can be greatly reduced. By the method, culture period is short, and costs for the culture mediums, labor, water and electricity can be saved. The method is suitable for industrialized seedling production of cymbidium, and the problems of low propagation efficiency, long period and the like in the prior art are solved.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a rapid propagation method for rhizome tissue culture of Chunlan. Background technique [0002] Chunlan, also known as Caolan, Pudilan, Duoxiang, etc., belongs to the ground species in the genus Orchid. Chunlan plants are small, with thin and narrow leaves, and the flower colors are usually green and yellow. There are purple-red stripes or spots on the perianth. Most of the spring orchids are rich in fragrance, elegant in color, beautiful in shape and elegant in leaf shape. They are unique species in the family of Orchidaceae and one of the main groups of orchids in my country. They are widely distributed. , rich in resources, and has a long history of cultivation. It has high ornamental value, medicinal value and collection value. Its market demand is very strong, wild Chunlan has been over-harvested, and wild natural resources have decreased sharply. It is one of the wild p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 方中明曾丽婷黄玮婷
Owner WUHAN INST OF BIOENG
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