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Double aqueous phase extraction method for separating 2,3-dihydroxybutane from fermentation liquor

A fermentation broth and butanediol technology, applied in the field of bioengineering, can solve the problems of low overall yield, high cost, and small separation capacity, and achieve the effects of short separation time, reduced cost, and simplified process

Inactive Publication Date: 2010-08-11
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for separating 2,3-butanediol from the fermentation broth by using a two-phase extraction method to solve the problems of low separation capacity, low overall yield, and high cost in the separation of 2,3-butanediol from the fermentation broth. 2,3-Butanediol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The fermentation broth was treated with a cellulose acetate ultrafiltration membrane with a cut-off molecular weight of 5,000 Daltons and an effective area of ​​1.5 m2 (product of Japan NIPRO Company, model FB-150AGA) to obtain a clarified filtrate. Add tripotassium phosphate trihydrate to the filtrate to form a solution containing 600 g / L of tripotassium phosphate, take 100 mL of this salt-containing fermentation broth, add 40 mL of 95% ethanol, mix, stand still, and separate phases to obtain 66 mL of the extract phase , raffinate phase 74mL. The partition coefficient of 2,3-butanediol was 30.8, and the recovery rate was 96.5%.

Embodiment 2

[0022] Take 100mL of fermentation broth, add 80g of dipotassium hydrogen phosphate, add 50mL of methanol after the salt is dissolved, mix and let it stand, the system is divided into three phases. The volume of the upper phase is 65mL, and the content of 2,3-butanediol is relatively high; the middle phase is mainly bacteria and protein, and the volume is 35mL; the concentration of salt in the lower phase is high, and the volume is 75mL. The middle phase was taken out and centrifuged at a low speed at 3000 r / min, and then divided into three phases. The upper phase had a higher content of 2,3-butanediol, with a volume of 13 mL; the middle phase was bacteria and protein, with a volume of 40 mL; The salt concentration in the phase was higher and the volume was 17 mL. The two upper phases were combined to obtain 78 mL of the clear liquid, which was the extract phase, and the partition coefficient of 2,3-butanediol was 7.78. The recovery rate was 86.8%.

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Abstract

The invention discloses a double-water phase extracting technique of 2, 3-butanediol from ferment liquid in the biological engineering technical domain, which is characterized by the following: adding inorganic salt and hydrophilic organics in the ferment liquid of 2, 3-butanediol to form new double-water phase; extracting to separate 2, 3-butanediol from ferment liquid.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the separation technology of microbial fermentation liquid, in particular to a method for separating 2,3-butanediol from the fermentation liquid. Background technique [0002] 2,3-butanediol is a valuable liquid fuel and can be used to manufacture important industrial organic solvent methyl ethyl ketone; it can also be used to produce 2-butene and 1,3-butadiene to produce artificial rubber 2,3-butanediol in the form of esterification is a precursor for the synthesis of polyimine, which can be applied to medicines, cosmetics, etc.; the diacetylated form of 2,3-butanediol obtained by catalytic dehydrogenation Alcohol can be used as a food additive with high-value spices; 2,3-butanediol itself can be used as a monomer to synthesize polymer compounds; the left-handed form of 2,3-butanediol can be used due to its lower freezing point As an antifreeze agent; in addition, 2,3-buta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C29/86C07C31/20
Inventor 修志龙李志刚张江洪孙亚琴张代佳
Owner DALIAN UNIV OF TECH
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