Double aqueous phase extraction method for separating 1,3-dihydroxypropane from fermentation liquor

A fermentation broth and propylene glycol technology, applied in the field of bioengineering, can solve the problems of complex separation process, low overall yield, and small separation capacity, and achieve the effects of simplified process, short separation time and reduced cost

Active Publication Date: 2007-08-08
DALIAN UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for extracting 3-propanediol from the fermentation broth by using a two-phase extraction method for the existing complex separation process, small separation capacity, low overall yield and high cost from the fermentation broth. Separation of 1,3-propanediol from

Method used

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Experimental program
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Effect test

Embodiment 1

[0020] With a cut-off molecular weight of 5,000 Daltons and an effective area of ​​1.5m 2 A cellulose acetate ultrafiltration membrane (product of Japan NIPRO Company, model FB-150AGA) was used to process the fermentation broth to obtain a clarified filtrate. Take 100mL of filtrate, add 70g of tripotassium phosphate trihydrate, and dissolve in salt to obtain 127ml of solution, then add 50mL of 95% ethanol, mix, stand still, and separate phases to obtain 87mL of extract phase and 88mL of raffinate phase. The partition coefficient of 1,3-propanediol was 6.29, and the recovery rate was 86.2%.

Embodiment 2

[0022] Take 2100mL of fermentation broth, add 750g of ammonium sulfate, and then add 1100mL of 95% ethanol after the salt is dissolved, mix and let it stand, the system is divided into three phases. The volume of the upper phase is 1200mL, and the content of 1,3-propanediol is high; the middle phase is mainly bacteria and protein, and the volume is 300mL; the salt concentration of the lower phase is high, and the volume is 2000mL. The middle phase was taken out and centrifuged at a low speed at 3000 rpm, and then divided into three phases. The upper phase contained 1,3-propanediol with a higher content and a volume of 130 mL; the middle phase was bacteria and protein with a volume of 40 mL; the lower phase contained The salt concentration is higher and the volume is 130mL. The two upper phases were combined to obtain 1330 mL of clear liquid, which was the extract phase, and the partition coefficient of 1,3-propanediol was 1.84. The two lower phases were combined, 1000 mL of 9...

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Abstract

The invention discloses a double-water phase extracting technique of 1, 3-propanediol from ferment liquid in the biological engineering technical domain, which is characterized by the following: adding inorganic salt and hydrophilic organics in the ferment liquid of 1, 3-propanediol to form new double-water phase; extracting to separate 1, 3-propanediol from ferment liquid.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the separation technology of microbial fermentation liquid, in particular to a method for separating 1,3-propanediol from the fermentation liquid. Background technique [0002] 1,3-propanediol is an important chemical raw material, which has the same purpose as ethylene glycol, 1,2-propanediol and 1,4-butanediol, but it is a new type of polyester material synthesized with terephthalic acid— — Polytrimethylene terephthalate (PTT) has excellent characteristics that many polyester materials do not have, such as nylon-like elastic recovery, anti-ultraviolet, ozone and nitrogen oxide coloring, low static electricity, low water adsorption, Good continuous printing and biodegradability without adding any special chemicals in the full color range. All of these show the bright application prospect of 1,3-propanediol, but its high price hinders its application. Since the production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/86C07C31/20
Inventor 修志龙李志刚江波孙亚琴张代佳
Owner DALIAN UNIV OF TECH
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