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Bone material preserving fluid and its prepn and use

A bone material and preservation solution technology, applied in the field of bone material preservation solution and its preparation, can solve the problems of bone graft material pollution, bone graft failure, bone graft material fracture, etc., achieve good tissue compatibility, increase antibacterial performance, Recipe Simple Effects

Inactive Publication Date: 2007-08-22
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of incompletely rewarmed frozen bones and incompletely hydrated freeze-dried bones may cause fracture of bone graft materials; at the same time, cumbersome operations are likely to cause contamination of bone graft materials, resulting in failure of bone grafts

Method used

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  • Bone material preserving fluid and its prepn and use
  • Bone material preserving fluid and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 Preparation of bone material preservation solution of the present invention

[0054] Table 1 Preparation formula of bone material preservation solution

[0055] Configure the bone material preservation solution A, B, C, D, E of the present invention according to the formula in Table 1.

[0056] The configuration steps are as follows:

[0057] 1. Take the formula amount of the culture-based container that can promote the growth and proliferation of osteoblasts, and dissolve it with pure water;

[0058] 2. Dissolve the tobramycin in the formula amount respectively;

[0059] 3. Dissolve vitamin C in the recipe amount;

[0060] 4. Dissolve the dexamethasone of the formula amount in it respectively;

[0061] 5. Dissolve the β-glycerophosphate sodium in the formula amount respectively;

[0062] 6. Filter and sterilize with Millipall decompression filters with a filter membrane pore size of 0.22um respectively, and seal and store for lat...

Embodiment 2

[0063] Embodiment 2 Preparation of bone material of the present invention

[0064] Preparation of bone material: take fresh allogeneic bone or xenogeneic bone (such as bovine bone, pig bone, etc.), remove attached soft tissue, cartilage, bone marrow, rinse with distilled water, and treat with 10% hydrogen peroxide at room temperature for 48-72 hours , change the medium once every 24 hours, rinse with distilled water, treat with ethanol at room temperature for 8 hours, rinse with distilled water, and process it into 6×0.5×0.5cm bone material according to the required specifications, at -40°C for 10 -5 Pa was freeze-dried for 24 hours, sterilized by 25KGy γ-ray irradiation, and every two pieces of materials were sealed into 1 package with a double-layer medical plastic bag. Material preservation solution A, B, C, D, E until the material is completely soaked, heat-sealed, and stored at 4°C.

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Abstract

The present invention relates to one kind of bone material preserving fluid and its preparation and use. The bone material preserving fluid consists of osteoblast culture medium in 10-15g / L, sodium beta-glycerophosphate in 8-12 mmol / L, dexamethasone in 0.02- 0.08 mmol / L, vitamin C in 10-100 mg / L, tobramycin in 10<4>-10<6> U / L or tetracycline in 4-12 mg / L, and water as the solvent. The bone material preserving fluid is used in preserving bone repairing material, especially biologically derived bone material, at normal temperature. It can maintain the physical and chemical performances of bone material, enhance its antibiotic performance and cell compatibility, and raise its atopic osteoplastic effect and bone defect repairing effect.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a bone material preservation solution and its preparation method and application. Background technique [0002] For bone defects caused by trauma, infection, tumor or congenital diseases, it is a common clinical method to repair bone defects with allogeneic bone or xenograft bone graft. There are two main types of preservation methods for bone materials reported so far: one is wet preservation, that is, soaking in preservation solution; the other is low-temperature preservation, and the third is freeze-drying and preservation at room temperature. [0003] The preservatives used in wet preservation reported so far include: honey, whole blood or plasma, mercuric sulfate, alcohol, formalin, chlorhexidine glycerol, dimethyl sulfoxide, etc., some of which require cryopreservation Cooperate use, use loaded down with trivial details, also can not overcome the shortcoming...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 罗静聪杨志明
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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