Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Black purple Tuowusu A and medical application in restraining grampostive bacteria

A gram-positive, black violet technology, applied in the field of medicine, can solve the problems of chemical composition research and biological activity research that are rarely reported.

Inactive Publication Date: 2010-12-08
WENZHOU MEDICAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the chemical constituents and biological activity of Ligularia sativa

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Black purple Tuowusu A and medical application in restraining grampostive bacteria
  • Black purple Tuowusu A and medical application in restraining grampostive bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Preparation of black purple argosin A

[0018] 1.1 Instruments and reagents

[0019] The melting point was measured with a micro-melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on a Polax-2L automatic polarimeter produced in Japan; the infrared spectrum (IR) was measured by a BrukerVector-22 infrared spectrometer, and pressed by KBr; The ultraviolet spectrum was measured with a Shimadzu UV-240 ultraviolet spectrophotometer; the hydrogen nuclear magnetic resonance spectrum ( 1 H-NMR), carbon nuclear magnetic resonance spectrum ( 13 C-NMR) and 2D NMR were measured by INOVA type superconducting nuclear magnetic resonance spectrometer (VARIAN INOVA-400MHz) (tetramethylsilyl ether was internal standard); electrospray mass spectrometry (ESI-MS) was measured by Bruker Esquire3000+ mass spectrometer, column Silica gel for chromatography (100-200, 200-300, 300-400 mesh) and ...

Embodiment 2

[0029] Example 2: Detection of Compounds of Formula (1) Inhibiting Gram-positive Bacteria

[0030] 2.1 Principle:

[0031] The "tube-and-disk method" is used to study the in vitro antibacterial activity of the test drug, which is to use the test drug added in the Oxford cup to diffuse into the medium inoculated with the test bacteria, thereby inhibiting the growth of the bacteria. The diameter of the bacterial circle was used to determine the antibacterial activity of the test drug.

[0032] 2.2 Detection of bacteria:

[0033] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0034] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0035] 2.3 Test drugs:

[0036] DMSO: DMSO solvent control;

[0037] Norfloxacin: 1.25mg / ml;

[0038] Compound of formula (1): 5.0 mg / ml; the sample was dissolved in DMSO.

[0039] 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention relates to Ligulatrovine A separated from Ligularia actroviolacea that can prevent and treat diseases related to Staphylococcus aureus and beta hemolytic streptococcus, its pharmaceutical salts and drug composition. Ligulatrovine A has significant inhibitive effect on Gram-positive bacteria, and can prevent and treat diseases such as boil, pustule, pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis, cystitis, pelvic inflammation, urinary inflammation, prostatitis, bacteremia, or abscess in muscle, skin, urogential region or central nervous system caused by Gram-positive bacteria infection.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to a trans-butene black ligusin A and Its pharmaceutically acceptable salts and pharmaceutical compositions. The sesquiterpene has the effect of obviously inhibiting the growth of Staphylococcus aureus and beta-hemolytic streptococcus, and can be expected to be used to prevent boils, pustules, pneumonia, osteomyelitis, acute myocarditis, and endocarditis caused by these two infections , meningitis, mastitis, cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis, cervicitis, and muscle, skin, urogenital , Central nervous system abscess. technical background [0002] Staphylococcus aureus (Staphylococcus aureus, referred to as "Staphylococcus aureus") is a Gram-positive coccus, and methylcillin-resistant Staphylococcus aureus (MRSA) is a strain th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C65/24A61K31/192A61P31/04A61K36/28
Inventor 黄可新赵军施树云马建设王彩芳王晓雨董南赵昱
Owner WENZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com