Human STRtyper PCR amplification fluorescence detection reagent kit
A detection kit and fluorescence enhancement technology, which can be used in measurement devices, determination/inspection of microorganisms, material analysis by optical means, etc., and can solve problems such as poor applicability, high price, and interference.
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Embodiment 1
[0033] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-20Gkit, which consists of the following components:
[0034] STR PCR reaction mixture 1.1ml×1 tube;
[0035] STRtyper primer mix 0.55ml×1 tube;
[0036] HS-Taq DNA polymerase 120μl×1 tube;
[0037] STR control DNA 0.3ml×1 tube;
[0038] STRtyper allele mixture 50μl×1 tube;
[0039] Internal standard 0.5ml×1 tube;
[0040] 310 spectral calibration standard 30μl×1 tube;
[0041] 3100 Spectral Calibration Standard 30μl×1 tube;
[0042] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is O-500ILS, and the fragment leng...
Embodiment 2
[0096] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-16GCkit, which consists of the following components:
[0097] STR PCR reaction mixture 1.1ml×1 tube;
[0098] STRtyper primer mix 0.55ml×1 tube;
[0099] HS-Taq DNA polymerase 120μl×1 tube;
[0100] STR control DNA 0.3ml×1 tube;
[0101] STRtyper allele mixture 50μl×1 tube;
[0102] Internal standard 0.5ml×1 tube;
[0103] 310 Spectral Calibration Standard 30μl×1 tube;
[0104] 3100 spectral calibration standard 30μl×1 tube;
[0105] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is R-500ILS or O-500ILS, and the ...
Embodiment 3
[0139] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-10F / Gkit, which consists of the following components:
[0140] STR PCR reaction mixture 1.1ml×1 tube;
[0141] STRtyper primer mix 0.55ml×1 tube;
[0142] HS-Taq DNA polymerase 120μl×1 tube;
[0143] STR control DNA 0.3ml×1 tube;
[0144] STRtyper allele mixture 50μl×1 tube;
[0145] Internal standard 0.5ml×1 tube;
[0146] 310 Spectral Calibration Standard 30μl×1 tube;
[0147] 3100 spectral calibration standard 30μl×1 tube;
[0148] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is R-500ILS or O-500ILS, and the...
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