Human STRtyper PCR amplification fluorescence detection reagent kit

A detection kit and fluorescence enhancement technology, which can be used in measurement devices, determination/inspection of microorganisms, material analysis by optical means, etc., and can solve problems such as poor applicability, high price, and interference.

Active Publication Date: 2010-05-19
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, only two American companies, PE and Promega, have successively launched STR genotype detection kits abroad, but this type of STR genotype detection kit is mainly designed for the STR loci of the Caucasian population. The applicability of the STR genotype detection of the yellow race is relatively poor, and the price is expensive. The use of imported reagents to establish the Chinese criminal DNA database is also susceptible to interference from external factors.

Method used

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  • Human STRtyper PCR amplification fluorescence detection reagent kit
  • Human STRtyper PCR amplification fluorescence detection reagent kit
  • Human STRtyper PCR amplification fluorescence detection reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-20Gkit, which consists of the following components:

[0034] STR PCR reaction mixture 1.1ml×1 tube;

[0035] STRtyper primer mix 0.55ml×1 tube;

[0036] HS-Taq DNA polymerase 120μl×1 tube;

[0037] STR control DNA 0.3ml×1 tube;

[0038] STRtyper allele mixture 50μl×1 tube;

[0039] Internal standard 0.5ml×1 tube;

[0040] 310 spectral calibration standard 30μl×1 tube;

[0041] 3100 Spectral Calibration Standard 30μl×1 tube;

[0042] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is ​​a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is O-500ILS, and the fragment leng...

Embodiment 2

[0096] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-16GCkit, which consists of the following components:

[0097] STR PCR reaction mixture 1.1ml×1 tube;

[0098] STRtyper primer mix 0.55ml×1 tube;

[0099] HS-Taq DNA polymerase 120μl×1 tube;

[0100] STR control DNA 0.3ml×1 tube;

[0101] STRtyper allele mixture 50μl×1 tube;

[0102] Internal standard 0.5ml×1 tube;

[0103] 310 Spectral Calibration Standard 30μl×1 tube;

[0104] 3100 spectral calibration standard 30μl×1 tube;

[0105] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is ​​a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is R-500ILS or O-500ILS, and the ...

Embodiment 3

[0139] The human STRtyper PCR amplification fluorescence detection kit in this example is STRtyper-10F / Gkit, which consists of the following components:

[0140] STR PCR reaction mixture 1.1ml×1 tube;

[0141] STRtyper primer mix 0.55ml×1 tube;

[0142] HS-Taq DNA polymerase 120μl×1 tube;

[0143] STR control DNA 0.3ml×1 tube;

[0144] STRtyper allele mixture 50μl×1 tube;

[0145] Internal standard 0.5ml×1 tube;

[0146] 310 Spectral Calibration Standard 30μl×1 tube;

[0147] 3100 spectral calibration standard 30μl×1 tube;

[0148] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer in which MgCl is dissolved 2 and deoxytriphosphate nucleotides; the STRtyper primer mix is ​​a 10× concentrate that includes fluorescein-labeled and unlabeled primers; the HS-Taq DNA polymerase concentration is 5U / μl ; The concentration of the STR control DNA is 10ng / μl; the internal standard is R-500ILS or O-500ILS, and the...

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PUM

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Abstract

The invention discloses a human STRtyper PCR enlarged fluoroscopic examination reagent kit with quick speed detecting, accuracy, good repeatability, good versatility, low price and practicality. The applicability of the reagent kit for Chinese is remarkable in particular. The serial products of the invention comprise the three following kits: (1) a STRtyper-20G kit with the largest STR gene locusof the global and most powerful recognizing ability; (2) a STRtyper-16GC kit which is more suitably used to build a criminal DNA database of Chinese population and identify the identity; (3) a STRtyper-10F / G kit with brand new STR gene locus. Each kit product comprises the component of 1.1 ml * 1 tube of STR PCR reaction mixture; 0.55 ml * 1 tube of STRtyper primer mixture; 120 microlitre * 1 tube of HS-Taq DNA polymerase; 0.3ml * 1 tube of STR control DNA; 50 microlitre * 1 tube of STRtyper allelomerphic gene mixture; 0.5 ml * 1 tube of internal standard; 30 microlitre* 1 tube of 310 spectrum correcting standard; 30 microlitre * 1 tube of 3100 spectrum correcting standard.

Description

technical field [0001] The invention relates to a human gene detection kit, in particular to a human STRtyper PCR amplification fluorescence detection kit. Background technique [0002] Human genomic DNA has 3 × 10 9 bp, 10% of which are tandem repeats, called satellite DNA. According to the length of the repeating unit, it can be divided into large satellites, medium satellites, small satellites and microsatellites. The repeat unit is only composed of 2-7bp called microsatellite, also known as short tandem repeat (Short Tandem Repeat, referred to as STR). The number of repeating units of microsatellite DNA in different human genomes is variable, thus forming extremely complex allelic fragment length polymorphisms. According to this law, a new STR genotyping technology has been established in recent years, which can identify different individuals and perform genetic analysis between individuals, which can be used for forensic science, archaeology, genetics and oncology. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/00C12Q1/68
Inventor 张兹钧
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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