Application of aza crown ether complex as nucleic acid cutting reagent

Abstract

The present invention pertains to the technical filed of biochemistry and molecular biology, in particular to the application of a kind of azacrown ether complexes as the nucleic acid cutting reagents. The azacrown ether complexes are formed by coordination of azacrown ether and metal ion, the azacrown ether is 1,12-diaza-3,4,9,10-dibenzo-5,8-diazacyclopentadecane-N,N'-diacetic acid, the metal ion can beany one of Cu<2+>, Ni<2+>, Co<2+> or Zn<2+> and so on. The invention can adopt the agarose gel electrophoresis to detect the cutting results of nucleic acid cutting reagents; the present invention provides a good basis and an effective route for the further synthetic DNA fixed point identification and the study of cutting reagents, which has great significance.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and molecular biology, and in particular relates to the application of a class of azacrown ether complexes in the preparation of nucleic acid cutting reagents. Background technique [0002] Artificial nucleic acid cleavage reagents, especially site-specific cleavage reagents, have important application value in the fields of biochemistry and molecular biology, and related research has been very active for more than 20 years. The base sequence recognized by natural nucleic acid restriction endonuclease is very short, only 4-8 base pairs, so when a longer nucleic acid chain is cut by restriction endonuclease, a lot of fragments will be generated , these fragments are difficult to be further utilized, so it is particularly important to find new nucleic acid cutting reagents. [0003] Synthetic nucleases represent a rapidly developing and challenging new field of chemical biology. Due to the sp...

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Problems solved by technology

[0004] Azacrown ether complexes are similar in structure to macrocyclic polyamine complexes, but from existing research data, azacrown ether complexes are not as good as macrocyclic polyamines in cutting effect on nucleic acids (Thorsten Berg, Anton Simeonov, Kim D. Janda. J. Comb. Chem., 1999, 1: 96-100, 47. Anne Roigk, Olga V. Yescheulova, Yury V. Fedorov, et al. Organic letters, 1999, 1: 833- 835, Ragunatnan K G, Schneider H J, Angew.Chem.Int.Ed.Engl., 1996, 35:1219-1221), so people's research on it is far less than that of macrocyclic polyamine complexes

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