Microbial feed additive capable of accelerating lactation and improving milk product quality and preparation method
A technology for microbial feed and lactation promotion, applied in animal feed, animal feed, food processing and other directions, can solve the problem of no outstanding novelty in the process, and achieve the effects of improving immunity, promoting development, and improving comprehensive disease resistance.
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Embodiment 1
[0028] The bacterial strains used in the production method of microbial feed additives that promote lactation and improve milk quality are all obtained through screening and optimization.
[0029] The method for oxygen fermentation and low-temperature culture, it comprises the following steps:
[0030] The first step. respectively optimize Lactobacillus plantarum, Streptococcus lactis, Lactobacillus casei, and Saccharomyces cerevisiae by using auxotrophic or ultraviolet-induced methods;
[0031] The second step. The above-mentioned strains are transferred into the broth culture medium respectively, and after cultivating at a temperature of 37°C for 24 hours, the activity titer is determined, and the selection growth is fast, and the number in the broth culture medium reaches 4 billion / More than 1 ml of strains are preserved;
[0032] Step 3. Transfer the bacterial strain into an eggplant bottle equipped with nutrient agar medium, cultivate it at a temperature of 37°C for 48 ...
Embodiment 2
[0039] Step 4. Inoculate Lactobacillus plantarum, Streptococcus lactis, and Saccharomyces cerevisiae in the steam-sterilized culture medium according to the ratio of 30%, 30%, and 40%, respectively. Flour 4%, molasses 0.5%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.1%, water 92.82%, put them into a mixer and stir, the stirring speed is 160r / min, the cultivation temperature is 37°C, the ventilation rate is 1:0.5, and the fermentation The time is 24 hours, and the stock solution of the compound bacterial agent is obtained;
[0040] Step 5. Add the stock solution of the compound bacterial agent into the culture medium and the nutrient carrier according to a specific ratio, and weigh it according to the following weight ratio, 12% of the strain stock solution, 22% of the culture medium, and 66% of the carrier, wherein, each component in the culture medium The proportions are: 30% bean cake powder, 25% corn flour, 15% starch, 5% yeast powder, 5% corn steep liquor, 4...
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