Human body cryoprotectant resurrection liquid
A cryoprotection and human body technology, applied in the preservation, application, and animal husbandry of human or animal bodies, it can solve the problems of cell damage and other problems, and achieve the effect of easy-to-obtain raw materials, low preparation cost, and reduce damage
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[0009] Formula 1: 22% dimethyl sulfoxide, 18% acetamide, 17% glycerol, 12% propylene glycol, 9% acetic acid, 8% n-propyl acetate, 5% amino acid, the balance is PBS liquid and conventional solvents, Dissolve powdered amino acid in PBS solution, then add dimethyl sulfoxide and mix well, then add other remaining materials, mix well, adjust the pH value to 8.0 with one equivalent of hydrochloric acid or sodium hydroxide to obtain a mixed solution, add sterilized Distilled water to obtain the protection and rejuvenation solution of the present invention, so that the concentration of the mixed solution in the protection and rejuvenation solution is 1.5 to 2.0 mol / L, bottled, and steam sterilized.
[0010] The preparation method of the PBS solution is as follows: 8 g of sodium chloride, 0.2 g of potassium chloride, 1.56 g of disodium hydrogen phosphate, and 0.2 g of potassium dihydrogen phosphate are poured into a beaker filled with distilled wat...
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