Human body cryoprotectant resurrection liquid

A cryoprotection and human body technology, applied in the preservation, application, and animal husbandry of human or animal bodies, it can solve the problems of cell damage and other problems, and achieve the effect of easy-to-obtain raw materials, low preparation cost, and reduce damage

Inactive Publication Date: 2008-07-30
郑葵飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prevent cells from being damaged and die during cooling, rewarming, and infiltration, it is required to add antifreeze during the cooling and rewarming procedures, which can improve the motility of cells after freezing and thawing, but the currently used refrigerants are still Can cause damage to 60% of cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] specific implementation plan

[0009] Formula 1: 22% dimethyl sulfoxide, 18% acetamide, 17% glycerol, 12% propylene glycol, 9% acetic acid, 8% n-propyl acetate, 5% amino acid, the balance is PBS liquid and conventional solvents, Dissolve powdered amino acid in PBS solution, then add dimethyl sulfoxide and mix well, then add other remaining materials, mix well, adjust the pH value to 8.0 with one equivalent of hydrochloric acid or sodium hydroxide to obtain a mixed solution, add sterilized Distilled water to obtain the protection and rejuvenation solution of the present invention, so that the concentration of the mixed solution in the protection and rejuvenation solution is 1.5 to 2.0 mol / L, bottled, and steam sterilized.

[0010] The preparation method of the PBS solution is as follows: 8 g of sodium chloride, 0.2 g of potassium chloride, 1.56 g of disodium hydrogen phosphate, and 0.2 g of potassium dihydrogen phosphate are poured into a beaker filled with distilled wat...

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PUM

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Abstract

The invention relates to a revivifier for cryonics protection, which comprises the following effective components with a weight percentage as: 19 percent to 23 percent of DMSO, 15 to 20 percent of acetamide, 15 to 19 percent of glycerol, 10 to 14percent of propanediol, 8 to 10 percent of acetic acid, 6 to 9 percent of propyl acetate and 4 to 6 percent of amino acid. The invention can remarkably reduce the cell damage in the process of freezing and rewarming.

Description

technical field [0001] The invention belongs to a cryoprotective rejuvenation liquid for human body. Background technique [0002] For cells, the cryopreservation methods that have achieved successful examples so far can be roughly divided into two categories, one is the "freezing" method, and the other is the "vitrification" method. The vast majority of successful cryopreservation is the application of the "freezing" method, and the "vitrification" method began in the 1980s, and has made important progress so far. In biological cells, water accounts for a large share, and most cells can only survive in aqueous solution. Thus, when the temperature drops below the freezing point of an aqueous solution, crystalline ice appears. At room temperature, cells and their solutions are in a state of osmotic equilibrium. When the temperature drops below the freezing point, the extracellular solution is first partially "frozen" out of ice; the concentration of the extracellular solut...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01N1/00
Inventor郑葵飞
Owner郑葵飞