Crotalaria sessiliflora test tube bulb inducement method

Abstract

The invention discloses a induction method of Brown Lily bulblet in vitro, including the following steps: (1)Brown Lily bulblet scale without pest is selected, then it is washed with tap water, dipped into 75% alcohol for 30 seconds, washed with sterile water, dipped into 3% sodium hypochlorite for 10 minutes, shaked for a while, washed with sterile water 6-7 times, then placed into culture dish containing asepsis filter paper and cut into blockes for reservation; (2) the base part of the scale is inoculated onto the induction medium for culture, the culture temperature is 23-27 DEG C, the light intensity is 1500Lx, the light time is 12 hours per day; (3) after 30-day induction culture, the grown bulblet scale is placed onto multiplication medium for multiplication culture, when the diameter of the bulblet scale is up to 1-2 cm, it is transplanted onto dish containing disinfected substrate, then watered and covered with fresh-keeping film, after 3-5 days it is ventilated, when new leaves are grown up on the tube seedling, the fresh-keeping film is removed. The invention can shorten the emerging time and reduce cost with simple operation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for inducing test tube bulbs of Guizhou wild lily (Liliumbrownii F.E.). Background technique [0002] Elegant in shape and pure in color, lilies are excellent materials for landscaping and home decoration. Lily bulbs are rich in nutrients such as protein, fat, starch, sugar, vitamins B1, B2 and vitamin C, and are a good tonic for strengthening the body. As the saying goes: "The mountain is not as high as Shengtang Mountain, and the taste is not as good as lily powder." Along with the development of human society and economy, lily has become a high-grade edible, medicinal and ornamental multi-purpose high-income economic crop. Guizhou's unique geographical environment and climatic conditions are rich in lily resources, among which wild lilies are distributed throughout the province. There are more than a dozen kinds of famous brand products made of Guizhou wild lily as raw...

AI Technical Summary

Problems solved by technology

The disinfection ability of mercury chloride is unquestionable, but it poses a great threat to the health of the experimental operators and the cleanliness of the environment, and it has a great corrosion on the experimental material lily bulbs. Whether the heavy metal mercury continues to exist in the lily test tube bulbs remains to be studied , and the price of mercury chloride is still very expensive

In short, while mercury chloride pollutes the environment, the explants usually appear discolored and rotted, which is not conducive to the formation of new bulbs.

The hormone composition and proportion of the medium are also important factors affecting the formation of wild lily bulbs. There are differences in the number of wild lily bulbs formed on the medium containing different hormones. The hormone proportions of the induction medium and the proliferation medium are different. Same, it needs to be separated when preparing, and the operation is complicated