Oral cavity squamous carcinoma diagnosis reagent, reagent kit and preventing and controlling medicament

A diagnostic reagent and technology for oral squamous cell carcinoma, which is applied in the field of tumor molecular biology, can solve the problems of drugs that have no oral squamous cell carcinoma, diagnostic reagents and kits that have no oral squamous cell carcinoma, and can improve the survival rate. , the effect of broad application prospects

Inactive Publication Date: 2008-08-20
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there are no relevant reports on diagnostic reagents and kits for early diagnosis of oral squamous cell carcinoma by detecting the expression l...

Method used

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  • Oral cavity squamous carcinoma diagnosis reagent, reagent kit and preventing and controlling medicament
  • Oral cavity squamous carcinoma diagnosis reagent, reagent kit and preventing and controlling medicament
  • Oral cavity squamous carcinoma diagnosis reagent, reagent kit and preventing and controlling medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preliminary determination of proteins related to the occurrence and development of oral squamous cell carcinoma

[0047] In this experiment, the classic method of differential proteomics research was used to analyze the protein expression profiles of 20 cases of surgically resected fresh oral squamous cell carcinoma tissues and their adjacent normal tissues using two-dimensional electrophoresis-tandem mass spectrometry-bioinformatics as the core proteomics technology , high-throughput identification of differentially expressed proteins associated with tumors. The difference points were selected by the PDQuest analysis software and quantitatively analyzed, and the results were obtained: the protein spots separated by two-dimensional electrophoresis were analyzed by the difference software, and 134 difference points with repeated expression and a difference of more than 1.5 times were obtained. ESI-Q-TOF mass spectrometry successfully identified 120 protein spot...

Embodiment 2

[0048] Example 2 Further determination of RACK1 as a protein related to the occurrence and development of oral squamous cell carcinoma

[0049] 1. Cell line

[0050] Oral tongue cancer cell line Tca8113 was preserved by West China Stomatological Hospital, human immortalized oral keratinized epithelial cell line HOK16E6E7 was donated by Xuan Lin (Charles R.Drew Uni.of Medicine, USA)

[0051] 2. Main reagents

[0052] RT-PCR: AccessQuick TM RT-PCR System, purchased from Promega Company.

[0053] The RACK1 rabbit polyclonal antibody used in Western blotting and the goat anti-rabbit secondary antibody were purchased from Santa Crus.

[0054] 3. Extraction of total cellular RNA for one-step semi-quantitative RT-PCR

[0055] Equal amounts of total RNA from Tca8113 cells and HOK16E6E7 cells were extracted, and then one-step semi-quantitative RT-PCR was performed. The primer design is shown in Table 1, and the b-actin gene was used as an internal reference.

[0056] Table 1 One-...

Embodiment 3

[0063] Example 3 Differential expression of RACK1 in normal tissue, OLK tissue, and OSCC tissue

[0064] 1. Tissue samples and reagents

[0065] 48 cases of OLK (oral leukoplakia tissue) and 76 cases of OSCC paraffin specimens were collected from the pathology department of West China Stomatological Hospital in 2005-2006. The clinicopathological data were obtained from medical records and histopathological reports, as shown in Table 2-1. 20 cases were normal Tissues were discarded from oral and maxillofacial plastic surgery.

[0066] The RACK1 rabbit polyclonal antibody used in immunohistochemistry, the goat anti-rabbit secondary antibody was purchased from Santa Crus, and the SABC kit was purchased from Wuhan Boster Company.

[0067] 2. Immunohistochemical staining

[0068] All specimens were immunohistochemically stained by SABC method under the same conditions, and then SPSS 11.5 software was used to perform one-way analysis of variance for comparison between groups, and ...

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Abstract

The invention belongs to the field of the tumor molecular biological, particularly relating to an oral squamous cell carcinoma diagnostic reagent, kits and medicine. The invention solves the technical problem that the existing medicine and reagent can not early diagnose oral squamous cell carcinoma and can not prevent oral carcinogenesis effectively. The invention oral squamous cell carcinoma diagnostic reagent contains the reagent which can detect expression level of protein RACK1 (activated protein kinase C receptor) and its mRNA content. The oral squamous cell carcinoma kits can be obtained with regular technology. By using the method of screening oral squamous cell carcinoma medicine, the medicine can be screened for preventing oral squamous cell carcinoma medicine, for example: by screen, obtaining effective RACK1 gene and RNA interference molecular, moreover the RNA interference result first reveals the functions of the RNA interference molecular in oral neoplasms apoptosis regulation and oral neoplasms apoptosis treatment, with broad application prospect.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to a diagnostic reagent, kit and prevention and treatment medicine for oral squamous cell carcinoma. Background technique [0002] Oral squamous carcinoma (OSCC) is one of the most common malignant tumors of the head and neck. There are 300,000 new cases worldwide every year, and only 50% of them survive for more than 5 years. Early diagnosis of OSCC has a better prognosis; however, for advanced lesions, conventional surgical treatment, radiotherapy, and chemotherapy are not effective. Early diagnosis of OSCC and prevention of its recurrence are the key factors to improve the survival rate of squamous cell carcinoma patients. Therefore, how to detect OSCC lesions early and find effective drugs to treat advanced and recurrent patients are clinical problems that need to be solved urgently. [0003] RACK1 (receptor for activated C-kinase 1) (also known as guanylate-...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68G01N33/577C12Q1/68
Inventor 陈谦明黄灿华王智曾昕
Owner SICHUAN UNIV
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