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Water chestnut polysaccharide and its extracting method and pharmaceutical use

A water chestnut, polysaccharide technology, applied in the direction of drug combination, antitumor drug, pharmaceutical formulation, etc., can solve the problem that the activity has not been reported in the literature.

Inactive Publication Date: 2008-09-17
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous experiments have shown that the water extract of water chestnut has good anti-tumor activity, but the activity of water chestnut polysaccharides has not been reported in the literature.

Method used

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  • Water chestnut polysaccharide and its extracting method and pharmaceutical use
  • Water chestnut polysaccharide and its extracting method and pharmaceutical use
  • Water chestnut polysaccharide and its extracting method and pharmaceutical use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Weigh the skin of water chestnut and put it in the extractor, soak it with 8 times the volume of distilled water for 8 days, heat and boil for 3 hours, filter and then repeat the extraction twice with 3 times the volume of water, combine the dark extract, filter and concentrate To a relative density of 1.15-1.20 (50°C);

[0039] 2. Add 3 times the amount of 95% ethanol to precipitate, stir continuously, filter, fully dissolve the precipitate with water, and centrifuge at 3000rpm for 15min to remove insoluble impurities;

[0040] 3. Precipitate with 3 times the amount of 95% ethanol, let stand at 4°C for 15 hours, and centrifuge at 3000rpm for 15 minutes; dehydrate the precipitate with 95% ethanol and absolute ethanol in turn, and then remove the ethanol with ether, and place it in a container containing P 2 o 5 and NaOH in a vacuum desiccator to obtain crude polysaccharides;

[0041] 4. Crude polysaccharide purification

[0042] a, the crude polysaccharide is diss...

Embodiment 2

[0045]1. Weigh the skin of water chestnut and put it in the extractor, soak it with 9 times the volume of distilled water for 9 days, heat and boil for 3.5 hours, filter and then repeat the extraction twice with 4 times the volume of water, combine the dark extract, filter and concentrate To a relative density of 1.15-1.20 (50°C);

[0046] 2. Add 2 times the amount of 95% ethanol to precipitate, stir continuously, filter, fully dissolve the precipitate with water, and centrifuge at 3000rpm for 15min to remove insoluble impurities;

[0047] 3. Precipitate with 2 times the amount of 95% ethanol, let stand at 4°C for 18 hours, and centrifuge at 3000rpm for 15 minutes; dehydrate the precipitate with 95% ethanol and absolute ethanol in turn, and then remove the ethanol with ether, and place it in a container containing P 2 o 5 and NaOH in a vacuum desiccator to obtain crude polysaccharides;

[0048] 4. Crude polysaccharide purification

[0049] a, the crude polysaccharide is dis...

Embodiment 3

[0052] 1. Weigh the skin of water chestnut and put it in the extractor, soak it with 10 times the volume of distilled water for 10 days, heat and boil for 4 hours, then repeat the extraction once with 5 times the volume of water, combine the dark extract, filter and concentrate To a relative density of 1.15-1.20 (50°C);

[0053] 2. Add 2.5 times the amount of 95% ethanol to precipitate, stir continuously, filter, fully dissolve the precipitate with water, and centrifuge at 3000rpm for 15min to remove insoluble impurities;

[0054] 3. Precipitate with 2.5 times the amount of 95% ethanol, let stand at 4°C for 20 hours, and centrifuge at 3000rpm for 15 minutes; dehydrate the precipitate with 95% ethanol and absolute ethanol in turn, and then remove the ethanol with ether, and place it in a container containing P 2 o 5 and NaOH in a vacuum desiccator to obtain crude polysaccharides;

[0055] 4. Crude polysaccharide purification

[0056] a, the crude polysaccharide is dissolved,...

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Abstract

The invention relates to water caltrops polysaccharide, the extraction method and the usage, belonging to field of Chinese herbal medicine, which is characterized in comprising following preparation methods: crude polysaccharide is prepared and purified and dissolved and produced into 5% of sugar solution; volume ratio of chloroform and normal butyl alcohol solution is 1 to 0.2; crude polysaccharide solution, chloroform and normal butyl alcohol solution are mixed according to volume ratio of 2 to 5 : 1 and are added into a vessel for oscillating about 30 min and centrifuging 15 min; the aqueous phase at the upper layer is taken for storing; the chloroform phase and protein solution are thrown away; protein can be removed three times according to the above method before the polysaccharide is dried and decolored through adopting active carbon method. The water caltrops polysaccharide has the advantages of anti-tumor, obvious proliferation inhibition and apoptosis induction upon Hela and U251 through pharmacodynamics experiment showing.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine. Background technique [0002] Water caltrop is recorded in the Pharmacopoeia of the People's Republic of China (2005 Edition One) and the Chinese Medicine Dictionary. It is the fruit of the water caltrop plant of the water caltrop family. It has the effects of clearing away heat, quenching thirst, strengthening the spleen, nourishing qi and improving eyesight. Previous experiments have shown that the water extract of water chestnut has good anti-tumor activity, but the activity of water chestnut polysaccharides has not been reported in the literature. [0003] Malignant tumors have become one of the three major killers of human life. While seeking and using anticancer drugs, the medical community has discovered that many chemical anticancer drugs often affect normal cells when they act on target cells, but the genotoxicity of plant drugs is not very high. Obviously, it shows that Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K31/715A61P35/00A61P15/00C08B37/00A61K131/00
Inventor 牛凤兰
Owner JILIN UNIV
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