Microorganism renovation agent of water environment and preparation method thereof

A microbial repair and microbial technology, applied in the field of applied biology, can solve the problems of ineffective degradation of residual bait and feces, slow effect, and inability to adapt to the water environment, and achieve the effect of preventing aquatic animal diseases

Inactive Publication Date: 2008-09-24
YANCHENG XINGHAI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, microbial preparations have been used in aquatic products, but there are many problems, such as some microorganisms outside the water environment, these microorganisms cannot adapt to the water body environment in the water body, and canno

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Perform slant activation on freeze-dried strains or strains stored at 4°C. Bacillus subtilis CGMCC 1.504, Bacillus licheniformis CGMCC1.105, and Alcaligenes denitrification CGMCC1.768 were inoculated into nutrient broth agar medium for slant activation; Actinomyces CGMCC4.1040 was inoculated into starch agar medium for slant activation ; Photosynthetic bacteria CGMCC4.1187 inoculated to photosynthetic bacteria solid medium for slant activation.

[0043]2) Preparation of the first-level seed solution: pick a typical colony from the activated agar plate, and inoculate Bacillus subtilis CGMCC 1.504, Bacillus licheniformis CGMCC 1.105, and Alcaligenes denitrification CGMCC 1.768 to sterile pH7. 0 nutrient broth, actinomycetes CGMCC4.1040 were inoculated in sterilized starch liquid medium at 35°C, 150 rpm, overnight culture; photosynthetic bacteria CGMCC4.1187 was inoculated into photosynthetic bacteria liquid medium, 35°C, light Culture for 4 days, light intensity 1500L...

Embodiment 2

[0048] Follow these steps:

[0049] 1) Perform slant activation on freeze-dried strains or strains stored at 4°C. Bacillus subtilis CGMCC1.504, Bacillus licheniformis CGMCC1.105, Alcaligenes denitrification CGMCC1.768 were activated with nutrient broth agar medium; Actinomyces CGMCC4.1040 was activated with starch agar medium; photosynthetic bacteria Slant activation of CGMCC4.1187 with photosynthetic bacterial solid medium.

[0050] 2) Preparation of primary seed solution. Pick typical colonies from the activated agar plate, inoculate Bacillus subtilis CGMCC 1.504, Bacillus licheniformis CGMCC 1.105, and Alcaligenes denitrifying Bacillus CGMCC 1.768 into sterile pH7.5 nutrient broth, respectively, and release the lines. Bacterium CGMCC4.1040 was inoculated in sterilized starch liquid medium at 37°C, 200rpm, and cultured overnight; photosynthetic bacteria CGMCC4.1187 was inoculated in photosynthetic bacteria liquid medium, 37°C, light cultured for 7 days, light intensity 200...

Embodiment 3

[0055] The water environment microbial remediation agent containing biological enzymes prepared in the embodiment of the present invention is 400-500ml / mu or 400-500g / mu (once every 2 weeks) in aquaculture eutrophication and other organic matter-polluted ponds It can effectively degrade the residual bait, feces, animal and plant corpses, ammonia nitrogen, hydrogen sulfide, nitrite and other harmful substances in the pond, effectively purify the water quality, improve the aquaculture water environment, inhibit the growth of bacteria in patients, and promote the production of green aquatic products. Escort.

[0056] 1. Materials and methods

[0057] 1.1 Test materials: The juveniles of Penaeus chinensis were purchased from healthy breeding farms and were bred in the same batch with the same specifications. The microbial remediation agent is prepared according to the above method.

[0058] 1.2 Test method Before the start of the test, four shrimp ponds with a size of 10 mu were...

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Abstract

The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.

Description

technical field [0001] The invention belongs to the technical field of applied biology, and relates to a water environment microbial restoration agent and a preparation method thereof. Background technique [0002] With the emergence of intensive aquaculture, the breeding density has been greatly increased, resulting in the increase of residual bait and animal excrement in the water body. Under such environmental conditions, it promotes the proliferation of microorganisms (including pathogenic bacteria), and the content of COD and ammonia nitrogen rises. , Dissolved oxygen has declined, and the breeding environment has continued to deteriorate, causing the aquaculture industry to suffer huge losses. The impact of water quality factors on the success or failure of aquaculture and yield is becoming more and more noticeable. The water quality is good, the pathogenic bacteria are few, and the cultured aquatic animals grow naturally; but the water quality conditions are poor, th...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20
Inventor 储卫华
Owner YANCHENG XINGHAI FEED
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