Artificial insemination process of large yellow croaker

A technology for large yellow croaker and female fish, which is applied in fish farming, medical science, climate change adaptation, etc. It can solve the problems of poor stability of artificial insemination technology and low insemination hatching rate, so as to reduce blindness, improve insemination effect, and avoid artificial insemination. blindness effect

Inactive Publication Date: 2010-12-08
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an artificial insemination method for large yellow croaker in view of the problems of poor stability and low insemination hatching rate of the existing large yellow croaker artificial insemination technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Intensified cultivation of broodstock in the same pond: the broodstock were moved from the sea area to the indoor for nutritional strengthening one month in advance, of which the number of broodstock for natural spawning was 600, and the number of broodstock for artificial insemination was 18 with pectoral fins cut as marks, the ratio of male to female Both are 2:1. The weight of female fish is 327-453g; the weight of male fish is 280-358g. Broodstock breeding pond area 35m 2 , the water depth is 1.2m. Feed a sufficient amount of anchovies or oysters, the water temperature is 20-22°C, the salinity is 1.019-1.021, the light is 300-500lux, and the daily water change is 120%.

[0023] 2. "Coupling" of the induced spawning environment: a place about 1m away from the natural spawning induced spawning pool 10m away 3 The circular cement pool was used as the artificial insemination and oxytocin pool, and the natural spawning and oxytocin pool was surrounded by black plas...

Embodiment 2

[0028] Similar to Example 1, the difference lies in the parent fish marking method of artificially induced spawning when the brood fish are cultivated in the same pond, such as cutting the ventral fin, cutting the caudal fin or electronic marking.

Embodiment 3

[0030] Similar to Example 1, the difference lies in the distance between the natural spawning pool and the artificial insemination pool, which ranges from 2 to 200 m.

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PUM

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Abstract

The invention provides an artificial insemination method of large yellow croakers and relates to the field of artificial insemination of fish. The method comprises the following steps: intensively culturing parent fish in the same pool, labeling parent fish for artificial insemination, culturing the labeled parent fish together with reproductive parent fish in the same pool with nutrient enhancers, building a natural spawning / induced spawning pool, building an artificial insemination / induced spawning pool 2-200 m away from the natural spawning / induced spawning pool to allow the parent fish for artificial insemination to feel the sound and behavior of parent fish in the natural spawning pool, injecting oxytocin to mature parent fish for artificial insemination and reproductive parent fish at the same time, putting the mature parent fish for artificial insemination and reproductive parent fish in the natural spawning / induced spawning pool and the artificial insemination / induced spawningpool respectively after spawning inducement, selecting female fish in good parturient state in the artificial insemination / induced spawning pool according to the abdominal circumference, two hours before natural spawning and five hours after natural spawning, and performing artificial insemination.

Description

technical field [0001] The invention relates to artificial insemination of fish, in particular to an artificial insemination method of large yellow croaker. Background technique [0002] Large yellow croaker (Pseudosciaena crocea) is the fish with the largest marine aquaculture output in my country, and it is also one of the six most advantageous export aquaculture aquatic products in China. In recent years, research on the genetic improvement technology of large yellow croaker for the purpose of improving muscle quality, growth rate and disease resistance has been carried out by using various breeding techniques such as cross breeding, polyploid breeding and family building. Among them, stable artificial insemination operation is one of the important basic technologies. At present, in the on-site operation of large yellow croaker genetics and breeding research, there is a common phenomenon that the eggs are difficult to fertilize or the hatching rate of fertilization is lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A61D19/02
CPCY02A40/81
Inventor 毛勇苏永全王军谢仰杰
Owner XIAMEN UNIV
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